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Poly-dl-lactic acid coated Bioglass® scaffolds: toughening effects and osteosarcoma cell proliferation

  • Syntactic & Composite Foams
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Abstract

The fabrication and in vitro characterisation of 45S5 Bioglass®-derived scaffolds coated with poly-dl-lactic acid (PDLLA) are presented. Two types of scaffolds with different porosities were studied. The scaffold microstructure was analysed by scanning electron microscopy before and after polymer coating. Compressive strength tests were performed in as-fabricated condition and after 28 days of immersion in simulated body fluid. It was found that PDLLA-coated samples have higher compression strength compared with the uncoated samples. Human osteosarcoma cell line (HOS-TE85) was used to determine the biocompatibility of the scaffolds. Cell proliferation and differentiated functions were investigated by AlamarBlue™, alkaline phosphatase and osteocalcin assays. The scaffolds were maintained in cell culture for periods of up to 14 days. The combined effects of the scaffold microstructure (porosity, surface roughness and bioreactivity) and the polymeric coating induce favourable HOS cell behaviour. The results confirmed the biocompatibility of Bioglass®/PDLLA composite scaffolds for HOS cell culture and contribute to the understanding of the characteristics of these scaffolds for potential applications in bone tissue engineering.

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Acknowledgement

This work was funded by the EU IntraEuropean Marie Curie fellowship (No 24248).

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Authors and Affiliations

  1. School of Mechanical and Systems Engineering, Newcastle University, Claremont Road, Newcastle upon Tyne, NE1 7RU, UK

    Oana Bretcanu

  2. Institute of Biomaterials, University of Erlangen-Nuremberg, 91058, Erlangen, Germany

    Aldo R. Boccaccini

  3. Division of Biomaterials and Tissue Engineering, UCL Eastman Dental Institute, London, WC1X 8LD, UK

    Vehid Salih

Authors
  1. Oana Bretcanu
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  2. Aldo R. Boccaccini
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  3. Vehid Salih
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Corresponding authors

Correspondence to Aldo R. Boccaccini or Vehid Salih.

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Bretcanu, O., Boccaccini, A.R. & Salih, V. Poly-dl-lactic acid coated Bioglass® scaffolds: toughening effects and osteosarcoma cell proliferation. J Mater Sci 47, 5661–5672 (2012). https://doi.org/10.1007/s10853-012-6315-4

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  • DOI: https://doi.org/10.1007/s10853-012-6315-4

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