Journal of Insect Conservation

, Volume 17, Issue 5, pp 1071–1079

Non-lethal method of DNA sampling in euglossine bees supported by mark–recapture experiments and microsatellite genotyping

Authors

    • Laboratório de Genética Evolutiva de Himenópteros, Departamento de Genética e EvoluçãoUniversidade Federal de São Carlos
    • Laboratory of Socioecology and Social Evolution, Zoological InstituteUniversity of Leuven
  • Margarita M. López-Uribe
    • Department of EntomologyCornell University
  • Marcelo Cervini
    • Departamento de Ciências BiológicasUniversidade Estadual do Sudoeste da Bahia
  • Marco Antonio Del Lama
    • Laboratório de Genética Evolutiva de Himenópteros, Departamento de Genética e EvoluçãoUniversidade Federal de São Carlos
SHORT COMMUNICATION

DOI: 10.1007/s10841-013-9582-8

Cite this article as:
Oi, C.A., López-Uribe, M.M., Cervini, M. et al. J Insect Conserv (2013) 17: 1071. doi:10.1007/s10841-013-9582-8

Abstract

Non-lethal sampling methods are of great interest for conservation genetic studies to prevent the death of individuals in populations that are threatened or in decline. With this aim, we tested a non-lethal method of partial antennae removal for DNA sampling in two euglossine bee species: Euglossa cordata and Eulaema nigrita. We validated the survival of the individuals through mark–recapture experiments during 16 months. The quality and quantity of the tissue for DNA analysis was verified through amplification and genotyping of nine and eleven microsatellite loci, respectively. Our results from the mark–recapture experiments showed equal recapture rates of individuals with intact and removed antennae (E. cordata χ2 = 2.492, df = 1, p = 0.114; E. nigrita χ2 = 1.683, df = 1, p = 0.194). Microsatellite loci were successfully genotyped in 97.1 and 97.6 % of the E. cordata and E. nigrita individuals, respectively. Our results validate the feasibility of using antennae tissue for DNA genetic analysis without compromising the survival of individual bees.

Keywords

Tissue samplingAntennaeOrchid beesEuglossa cordataEulaema nigrita

Copyright information

© Springer Science+Business Media Dordrecht 2013