Journal of Assisted Reproduction and Genetics

, Volume 30, Issue 5, pp 623–631

Astaxanthin ameliorates heat stress-induced impairment of blastocyst development In Vitro: –astaxanthin colocalization with and action on mitochondria–

Authors

  • T. Kuroki
    • Laboratory of Animal Physiology and Functional AnatomyGraduate School of Agriculture, Kyoto University
    • Laboratory of Animal Physiology and Functional AnatomyGraduate School of Agriculture, Kyoto University
  • T. Okada
    • Animal Health DivisionASKA Pharmaceutical Co., Ltd
  • T. Maoka
    • Division of Food Function and ChemistryResearch Institute for Production Development
  • A. Kitamura
    • AstaReal Co., Ltd
  • M. Sugimoto
    • Laboratory of Animal Physiology and Functional AnatomyGraduate School of Agriculture, Kyoto University
  • S. Kume
    • Laboratory of Animal Physiology and Functional AnatomyGraduate School of Agriculture, Kyoto University
Embryo Biology

DOI: 10.1007/s10815-013-9987-z

Cite this article as:
Kuroki, T., Ikeda, S., Okada, T. et al. J Assist Reprod Genet (2013) 30: 623. doi:10.1007/s10815-013-9987-z

Abstract

Purpose

The effects of astaxanthin (Ax) on the in vitro development of bovine embryos cultured under heat stress were investigated in combination with the assessment of its cellular accumulation and action on mitochondrial membrane potential (ΔΨm).

Methods

Bovine ≥8-cell embryos were collected on day 3 after in vitro fertilization and exposed to single (day 4) or repeated (day 4 and 5) heat stress (10 h/day at 40.5 °C). Ax was added into culture medium under the repeated heat stress and blastocyst development was evaluated. The cellular uptake of Ax in embryos was examined using bright-field and confocal laser-scanning microscopy, and high-performance liquid chromatography. The relationship between Ax and mitochondria localization was assessed using MitoTracker dye. The effects of Ax on ΔΨm were investigated using JC-1 dye.

Results

Blastocyst development in the repeated heat stress treatment decreased significantly (P < 0.05) compared with those in single heat stress or normal thermal treatment. The addition of Ax into culture medium did lead to a significant recovery in blastocyst development in the repeated heat-treated group. Ax was detected in cytoplasm of embryos and observed to colocalize with mitochondria. Ax recovered ΔΨm in embryos that was decreased by the heat treatment.

Conclusions

Ax ameliorated the heat stress-induced impairment of blastocyst development. Our results suggest that the direct action of Ax on mitochondrial activity via cellular uptake is a mechanism of the ameliorating effects.

Keywords

Preimplantation embryosHeat stressAstaxanthinCellular uptakeMitochondria

Copyright information

© Springer Science+Business Media New York 2013