Journal of Assisted Reproduction and Genetics

, Volume 31, Issue 1, pp 79–88

Differences in cumulus cells gene expression between modified natural and stimulated in vitro fertilization cycles

Authors

  • Tanja Burnik Papler
    • Department of Human Reproduction, Division of GynecologyUniversity Medical Centre Ljubljana
  • Eda Vrtačnik Bokal
    • Department of Human Reproduction, Division of GynecologyUniversity Medical Centre Ljubljana
  • Klementina Fon Tacer
    • Department of PhysiologyUT Southwestern Medical Center
  • Peter Juvan
    • Centre for Functional Genomics and Bio-Chips, Institute of Biochemistry, Faculty of MedicineUniversity of Ljubljana
  • Irma Virant Klun
    • Department of Human Reproduction, Division of GynecologyUniversity Medical Centre Ljubljana
    • Department of Human Reproduction, Division of GynecologyUniversity Medical Centre Ljubljana
Assisted Reproduction Technologies

DOI: 10.1007/s10815-013-0135-6

Cite this article as:
Papler, T.B., Bokal, E.V., Tacer, K.F. et al. J Assist Reprod Genet (2014) 31: 79. doi:10.1007/s10815-013-0135-6

Abstract

Purpose

The aim of our study was to determine whether there are any differences in the cumulus cell gene expression profile of mature oocytes derived from modified natural IVF and controlled ovarian hyperstimulation cycles and if these changes could help us understand why modified natural IVF has lower success rates.

Methods

Cumulus cells surrounding mature oocytes that developed to morulae or blastocysts on day 5 after oocyte retrieval were submitted to microarray analysis. The obtained data were then validated using quantitative real-time PCR.

Results

There were 66 differentially expressed genes between cumulus cells of modified natural IVF and controlled ovarian hyperstimulation cycles. Gene ontology analysis revealed the oxidation-reduction process, glutathione metabolic process, xenobiotic metabolic process and gene expression were significantly enriched biological processes in MNIVF cycles. Among differentially expressed genes we observed a large group of small nucleolar RNA’s whose role in folliculogenesis has not yet been established.

Conclusion

The increased expression of genes involved in the oxidation-reduction process probably points to hypoxic conditions in modified natural IVF cycles. This finding opens up new perspectives for the establishment of the potential role that oxidation-reduction processes have in determining success rates of modified natural IVF.

Keywords

Cumulus cellsGene expressionStimulated IVFUnstimulated IVF

Supplementary material

10815_2013_135_MOESM1_ESM.doc (30 kb)
Table S1(DOC 30 kb)
10815_2013_135_MOESM2_ESM.doc (53 kb)
Table S2(DOC 53 kb)
10815_2013_135_MOESM3_ESM.doc (36 kb)
Table S3(DOC 35 kb)

Copyright information

© Springer Science+Business Media New York 2013