Fertilization, embryo development, and clinical outcome of immature oocytes obtained from natural cycle in vitro fertilization
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To analyze the fertilization, embryo development, and clinical outcome of immature oocytes obtained from natural cycle IVF in women with regular cycles.
Natural cycle IVF was performed in 28 patients who had normal ovaries, > 6 antral follicle counts and were less than 40 years old (n = 28 cycles). An hCG trigger of 10,000 IU was administered 36 h before oocyte collection when the diameter of the dominant follicle (DF) was over 12 mm. Oocytes were retrieved from DF as well as from the cohort of smaller follicles. Embryological aspects of the mature and immature oocytes retrieved from these cycles as well as the implantation and clinical pregnancy rates depending on the origin of the embryos transferred were evaluated.
Overall clinical pregnancy and implantation rates were 20.8 % and 6.7 %, respectively. There were no differences in in vitro maturation (IVM), fertilization and embryo development between immature oocytes retrieved with and without in vivo matured oocytes. However, the clinical and implantation rates in cycles with embryos produced from in vivo matured oocytes transferred were better than the cycles where only IVM embryos were transferred (30.8 %, 9.1 % vs. 9.1 %, 3.2 %).
Although our results show that immature oocytes from natural cycle IVF can fertilize normally and can be used to increase the number of embryos available for transfer, the embryos derived from the immature oocytes in natural cycles IVF have a poorer reproductive potential.
- Steptoe PC, Edwards RG. Birth after the reimplantation of a human embryo. Lancet. 1978;2(8085):366. CrossRef
- MacDougall MJ, Tan SL, Hall V, Balen A, Mason BA, Jacobs HS. Comparison of natural with clomiphene citrate-stimulated cycles in in-vitro fertilization: a prospective, randomized trial. Fertil Steril. 1994;61:1052–7.
- Janssens RM, Lambalk CB, Vermeiden JP, Schats R, Bernards JM, et al. In-vitro fertilization in a spontaneous cycle:easy, cheap and realistic. Hum Reprod. 2000;15:314–8. CrossRef
- Johnston I, Lopata A, Speirs A, Hoult I, Kellow G, du Plessis Y. In vitro fertilization: the challenge of the eighties. Fertil Steril. 1981;36:699–706.
- Lopata A, Brown JB, Leeton JF, Talbot JM, Wood C. In vitro fertilization of preovulatory oocytes and embryo transfer in infertile patients treatedwith clomiphene and human chorionic gonadotropin. Fertil Steril. 1978;30:27–35.
- Edwards RG. IVF, IVM, natural cycle IVF, minimal stimulation IVF – time for a rethink. Reprod Biomed Online. 2007;15:106–19. CrossRef
- Chian RC, Buckett WM, Abdul Jalil AK, Son WY, Sylvestre C, Rao D, et al. Natural-cycle in vitro fertilization combined with in vitro maturation of immature oocytes is a potential approach in infertility treatment. Fertil Steril. 2004;82:1675–8. CrossRef
- Chian RC, Lim JH, Tan SL. State of the art in in-vitro oocyte maturation. Curr Opin Obstet Gynecol. 2004;16:211–9. CrossRef
- Lim JH, Park SY, Yoon SH, Yang SH, Chian RC. Combination of natural cycle IVF with IVM as infertility treatment. In: Tan SL, Chian RC, Buckett WM, editors. In-vitro maturation of human oocytes: basic science to clinical application. London: Informa Healthcare Press; 2007. p. 353–60.
- Lim JH, Yang SH, Xu Y, Yoon SH, Chian RC. Selection of patients for natural cycle in vitro fertilization combined with in vitro maturation of immature oocytes. Fertil Steril. 2009;91:1050–5. CrossRef
- Yang SH, Patrizio P, Yoon SH, Lim JH, Chian RC. Comparison of pregnancy outcomes in natural cycle IVF/M treatment with or without mature oocytes retrieved at time of egg collection. Syst Biol Reprod Med. 2012;58:154–9. CrossRef
- Son WY, Tan SL. Laboratory and embryological aspects of hCG-primed in vitro maturation cycles for patients with polycystic ovaries. Hum Reprod Update. 2010;16:675–89. CrossRef
- Hyun CS, Cha JH, Son WY, Yoon SH, Kim KA, Lim JH. Optimal ICSI timing after the first polar body extrusion in in vitro matured human oocytes. Hum Reprod. 2007;22:1991–5. CrossRef
- Ginther OJ, Beg MA, Bergfelt DR, Donadeu FX, Kot K. Follicle selection in monovular species. Biol Reprod. 2001;65:638–47. CrossRef
- Barnes FL, Sirard MA. Oocyte maturation. Semin Reprod Med. 2000;18:123–31. CrossRef
- Chian RC, Chung JT, Downey BR, Tan SL. Maturational and developmental competence of immature oocytes retrieved from bovine ovaries at different phases of folliculogenesis. Reprod Biomed Online. 2002;4:127–32. CrossRef
- Thornton MH, Francis MM, Paulson RJ. Immature oocyte retrieval: lessons from unstimulated IVF cycles. Fertil Steril. 1998;70:647–50. CrossRef
- Son WY, Chung JT, Herrero B, Dean N, Demirtas E, Holzer H, et al. Selection of the optimal day for oocyte retrieval based on the diameter of the dominant follicle in hCG-primed in vitro maturation cycles. Hum Reprod. 2008;23:2680–5. CrossRef
- Paulson RJ, Sauer MV, Francis MM, Macaso T, Lobo RA. Factors affecting pregnancy success of human in-vitro fertilization in unstimulated cycles. Hum Reprod. 1994;9:1571–5.
- Fertilization, embryo development, and clinical outcome of immature oocytes obtained from natural cycle in vitro fertilization
Journal of Assisted Reproduction and Genetics
Volume 30, Issue 1 , pp 43-47
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- Dominant follicle
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- Natural cycle
- Normal ovary
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