Gamete Biology

Journal of Assisted Reproduction and Genetics

, Volume 29, Issue 7, pp 631-635

First online:

Supplementation of biotin to sperm preparation medium increases the motility and longevity in cryopreserved human spermatozoa

  • Guruprasad KalthurAffiliated withClinical Embryology, Division of Reproductive Medicine, Kasturba Medical College, Manipal UniversityClinical Embryology, Department of Obstetrics and Gynecology, Kasturba Medical College, Manipal University Email author 
  • , Sujith R SalianAffiliated withClinical Embryology, Division of Reproductive Medicine, Kasturba Medical College, Manipal University
  • , Farid KeyvanifardAffiliated withClinical Embryology, Division of Reproductive Medicine, Kasturba Medical College, Manipal University
  • , Shyam SreedharanAffiliated withClinical Embryology, Division of Reproductive Medicine, Kasturba Medical College, Manipal University
  • , Jeena S ThomasAffiliated withClinical Embryology, Division of Reproductive Medicine, Kasturba Medical College, Manipal University
  • , Pratap KumarAffiliated withClinical Embryology, Division of Reproductive Medicine, Kasturba Medical College, Manipal University
  • , Satish K AdigaAffiliated withClinical Embryology, Division of Reproductive Medicine, Kasturba Medical College, Manipal University

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Abstract

Purpose

To study the effect of supplementing biotin to sperm preparation medium on the motility of frozen-thawed spermatozoa.

Methods

Semen samples of men attending the University infertility clinic (n = 105) were cryopreserved using glycerol-egg yolk-citrate buffered cryoprotective medium in liquid nitrogen. After a period of two weeks, the semen samples were thawed and the motile spermatozoa were extracted by swim-up technique using Earle’s balanced salt solution (EBSS) medium supplemented with either biotin (10 nM) or pentoxifylline (1 mM). The post-wash motility was observed up to 4 h after incubation.

Results

Both biotin and pentoxifylline supplementation resulted in significant increase in total motility (p < 0.05), progressive motility (p < 0.001) and rapid progressive motility (p < 0.05 v/s biotin and p < 0.01 v/s pentoxifylline) compared to the control at 1 h post-incubation period. Significantly higher percentage of total (p < 0.01, p < 0.05 in biotin and pentoxifylline respectively), progressive (p < 0.001) and rapid progressive motilities (p < 0.01) were observed in these two groups even at 2 h compared to the control. In the control group at 4 h after incubation, ~11% decline in total motility and ~8% decline in progressive motility was observed. However, in both biotin and pentoxifylline group the motility was significantly higher than control (p < 0.001). No significant difference in the motility was observed between biotin and pentoxifylline groups at any of the time intervals studied.

Conclusions

Biotin can enhance the sperm motility and prolong the survival of frozen-thawed semen samples which may have potential benefit in assisted reproductive technology field.

Keywords

Biotin pentoxifylline spermatozoa cryopreservation post-thaw motility