Alginate scaffold for organ culture of cryopreserved-thawed human ovarian cortical follicles

  • Alon Kedem
  • Ariel Hourvitz
  • Benjamin Fisch
  • Michal Shachar
  • Smadar Cohen
  • Avi Ben-Haroush
  • Joshua Dor
  • Enrique Freud
  • Carmela Felz
  • Ronit Abir
Fertility Preservation

DOI: 10.1007/s10815-011-9605-x

Cite this article as:
Kedem, A., Hourvitz, A., Fisch, B. et al. J Assist Reprod Genet (2011) 28: 761. doi:10.1007/s10815-011-9605-x

Abstract

Purpose

To compare macroporous alginate scaffolds with Matrigel for culturing frozen-thawed human primordial follicles in organ culture.

Methods

Twelve girls/women donated ovarian tissue. One tissue sample was fixed immediately after thawing (uncultured samples). Slices were cultured for 2 weeks on either Matrigel or on alginate scaffolds with a serum-free culture medium. Growth evaluation consisted of follicular counts and classification, immunohistochemistry and measurement of 17β-Estradiol (E2) production.

Results

The number of developing follicles was significantly higher in alginate scaffold-cultured samples than on Matrigel with a concomitant decrease in the number of primordial follicles in alginate scaffold-cultured samples than uncultured samples. The number of atretic follicles after 1 week was significantly higher in the Matrigel-cultured samples than in the alginate scaffold cultured samples. E2 production was similar in both groups.

Conclusions

Three dimensional alginate scaffolds are a promising putative in vitro technology for developing human primordial follicles.

Keywords

Alginate scaffold 17β-Estradiol (E2Human primordial follicles Matrigel Proliferating cell nuclear antigen (PCNA) 

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Alon Kedem
    • 1
    • 2
  • Ariel Hourvitz
    • 2
  • Benjamin Fisch
    • 1
  • Michal Shachar
    • 3
  • Smadar Cohen
    • 4
  • Avi Ben-Haroush
    • 1
  • Joshua Dor
    • 2
  • Enrique Freud
    • 5
  • Carmela Felz
    • 1
  • Ronit Abir
    • 1
    • 6
  1. 1.Infertility and IVF Unit, Helen Schneider Hospital for Women, Rabin Medical Center, Beilinson Hospital, Petach Tikva and Sackler Faculty of MedicineTel Aviv UniversityTel AvivIsrael
  2. 2.Infertility and IVF Unit, Department of Obstetrics and Gynecology, Sheba Medical Center, Tel Hashomer, Ramat Gan and Sackler Faculty of MedicineTel Aviv UniversityTel AvivIsrael
  3. 3.Department of Chemical EngineeringSami Shamoon College of EngineeringBeer-ShevaIsrael
  4. 4.The Avram and Stella Goldstein-Goren Department of Biotechnology EngineeringBen-Gurion University of the NegevBeer-ShevaIsrael
  5. 5.Department of Pediatric Surgery, Schneider Children’s Medical Center of Israel, Rabin Medical Center, Petach Tikvah, Israel and Sackler School of MedicineTel Aviv UniversityTel AvivIsrael
  6. 6.Infertility and IVF Unit, Helen Schneider Hospital for Women, Rabin Medical Center, Beilinson HospitalPetach TikvaIsrael