Alginate scaffold for organ culture of cryopreserved-thawed human ovarian cortical follicles
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- Kedem, A., Hourvitz, A., Fisch, B. et al. J Assist Reprod Genet (2011) 28: 761. doi:10.1007/s10815-011-9605-x
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To compare macroporous alginate scaffolds with Matrigel for culturing frozen-thawed human primordial follicles in organ culture.
Twelve girls/women donated ovarian tissue. One tissue sample was fixed immediately after thawing (uncultured samples). Slices were cultured for 2 weeks on either Matrigel or on alginate scaffolds with a serum-free culture medium. Growth evaluation consisted of follicular counts and classification, immunohistochemistry and measurement of 17β-Estradiol (E2) production.
The number of developing follicles was significantly higher in alginate scaffold-cultured samples than on Matrigel with a concomitant decrease in the number of primordial follicles in alginate scaffold-cultured samples than uncultured samples. The number of atretic follicles after 1 week was significantly higher in the Matrigel-cultured samples than in the alginate scaffold cultured samples. E2 production was similar in both groups.
Three dimensional alginate scaffolds are a promising putative in vitro technology for developing human primordial follicles.