, Volume 28, Issue 9, pp 761-769
Date: 23 Jul 2011

Alginate scaffold for organ culture of cryopreserved-thawed human ovarian cortical follicles

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Abstract

Purpose

To compare macroporous alginate scaffolds with Matrigel for culturing frozen-thawed human primordial follicles in organ culture.

Methods

Twelve girls/women donated ovarian tissue. One tissue sample was fixed immediately after thawing (uncultured samples). Slices were cultured for 2 weeks on either Matrigel or on alginate scaffolds with a serum-free culture medium. Growth evaluation consisted of follicular counts and classification, immunohistochemistry and measurement of 17β-Estradiol (E2) production.

Results

The number of developing follicles was significantly higher in alginate scaffold-cultured samples than on Matrigel with a concomitant decrease in the number of primordial follicles in alginate scaffold-cultured samples than uncultured samples. The number of atretic follicles after 1 week was significantly higher in the Matrigel-cultured samples than in the alginate scaffold cultured samples. E2 production was similar in both groups.

Conclusions

Three dimensional alginate scaffolds are a promising putative in vitro technology for developing human primordial follicles.

Capsule Culturing human ovarian cortical tissue on a macroporous alginate scaffold seems to promote better follicular development than culturing on Matrigel