Journal of Assisted Reproduction and Genetics

, 26:47

The presence of bacteria species in semen and sperm quality

  • Elena Moretti
  • Serena Capitani
  • Natale Figura
  • Andrea Pammolli
  • Maria Grazia Federico
  • Valentina Giannerini
  • Giulia Collodel
ANDROLOGY

DOI: 10.1007/s10815-008-9283-5

Cite this article as:
Moretti, E., Capitani, S., Figura, N. et al. J Assist Reprod Genet (2009) 26: 47. doi:10.1007/s10815-008-9283-5

Abstract

Purpose

To verify the prevalence of semen bacterial contamination and whether the contamination could decrease sperm quality.

Methods

Spermiogram, semen culture, and sperm transmission electron microscopy (TEM) analysis were performed. TEM data were elaborated using a mathematical formula that calculates a fertility index (FI)—able to define patients as fertile or infertile—and the percentage of sperm apoptosis, immaturity and necrosis. We aligned the amino acid sequence of beta-tubulin with protein of the most frequent species isolated from semen.

Results

Patients were divided according to the contaminating species; in each group, we observed fertile individuals, in whom the semen quality was similar to that of controls and infertile men whose sperm quality was significantly decreased, in terms of motility, FI, apoptosis and necrosis. Partial homology between β-tubulin and bacterial proteins was observed.

Conclusion

Sperm bacterial contamination is quite frequent and could contribute to the deterioration of the sperm quality of infertile men.

Keywords

BacteriaSemen qualitySpermatozoaTEM

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Elena Moretti
    • 1
  • Serena Capitani
    • 2
  • Natale Figura
    • 3
  • Andrea Pammolli
    • 2
  • Maria Grazia Federico
    • 1
  • Valentina Giannerini
    • 1
  • Giulia Collodel
    • 1
  1. 1.Department of Biomedical Sciences, Biology Section, Interdepartmental Centre for Research and Therapy of Male InfertilityUniversity of SienaSienaItaly
  2. 2.Department of Physiopathology, Experimental Medicine and Public HealthUniversity of SienaSienaItaly
  3. 3.Department of Internal Medicine, Endocrine-Metabolic Sciences and BiochemistryUniversity of SienaSienaItaly