, Volume 25, Issue 2-3, pp 107-113
Date: 06 Feb 2008

Evaluation of the developmental competence and chromosomal compliment of mouse oocytes derived from in-vitro growth and maturation of preantral follicles

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access



To evaluate the developmental potential and aneuploidy rates of in-vitro versus in-vivo grown and matured mouse oocytes.


Mice were superovulated to obtain in-vivo matured oocytes. Mouse preantral follicles were also mechanically isolated and cultured in-vitro. In-vitro fertilization (IVF) was performed and fertilization, cleavage, and morula/blastocyst formation rates were compared between groups. Cytogenetic analysis was used to compare oocyte aneuploidy rates and aneuploidy characteristics in the developing embryos.


In-vivo oocyte maturation resulted in higher IVF fertilization, cleavage, and morula/blastocyst formation rates versus in-vitro follicle culture (96.4% versus 78.5%, p < 0.001; 95.3% versus 77.4%, p < 0.001; 94.1% versus 76.9%, p < 0.001). Total aneuploidy rates were higher in embryos derived from in-vitro matured oocytes versus those grown in-vivo (4.0% versus 1.3%, p < 0.05).


Results indicate a reduced developmental competency of in-vitro matured oocytes. The data also highlight an increased susceptibility to meiotic errors in early stage follicles undergoing in vitro culture.

Capsule Impaired developmental capacity and errors in proper chromosome segregation are observed in mouse oocytes generated from in-vitro preantral follicle culture versus those developed in-vivo.