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- Lobb, D.K. & Younglai, E.V. J Assist Reprod Genet (2006) 23: 93. doi:10.1007/s10815-006-9025-5
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Purpose: To validate an expedited method for the removal of erythrocytes when preparing IVF granulosa-luteal cells for culture.
Methods: Granulosa cells were enriched for culturing from follicular aspirates by density gradient centrifugation and by hypo-osmotic lysis treatments.
Results: Cells prepared by either method showed the same cell viability and produced progesterone in similar quantities.
Conclusions: Using hypo-osmotic lysis to remove erythrocytes does not impair granulosa cell viability or steroidogenesis. It avoids multiple density gradient centrifugations and washings, and yields IVF granulosa cells ready for culture efficiently.