Journal of Assisted Reproduction and Genetics

, Volume 23, Issue 2, pp 93–95


A simplified method for preparing IVF granulosa cells for culture

DOI: 10.1007/s10815-006-9025-5

Cite this article as:
Lobb, D.K. & Younglai, E.V. J Assist Reprod Genet (2006) 23: 93. doi:10.1007/s10815-006-9025-5

Purpose: To validate an expedited method for the removal of erythrocytes when preparing IVF granulosa-luteal cells for culture.

Methods: Granulosa cells were enriched for culturing from follicular aspirates by density gradient centrifugation and by hypo-osmotic lysis treatments.

Results: Cells prepared by either method showed the same cell viability and produced progesterone in similar quantities.

Conclusions: Using hypo-osmotic lysis to remove erythrocytes does not impair granulosa cell viability or steroidogenesis. It avoids multiple density gradient centrifugations and washings, and yields IVF granulosa cells ready for culture efficiently.


Cell culturegranulosa-lutealhypotonic lysisIVF cells

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  1. 1.Department of Obstetrics and GynecologyMcMaster UniversityHamiltonCanada