, Volume 23, Issue 3, pp 111-119
Date: 19 Apr 2006

Heparin-binding epidermal growth factor (HB-EGF) may improve embryonic development and implantation by increasing vitronectin receptor (integrin ανβ3) expression in peri-implantation mouse embryos

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Purpose: This study investigated the effects of HB-EGF on expression of integrin \(\alpha\nu\beta\hbox{{\it 3}}\) and implantation of embryos.

Methods: Two-cell embryos were recovered and cultured with or without 10 ng/mL HB-EGF for 96h. Expression of integrin \(\alpha\nu\beta\hbox{{\it 3}}\) in cultured embryos was examined by real time-RT-PCR and immunofluorescence analysis; embryos were cultured with or without HB-EGF, then transferred into the uteri of pseudo-pregnant female mice in order to analyze their implantation rate.

Results: HB-EGF improved embryonic hatching and outgrowth during extended culture, and up-regulated expression of integrin \(\alpha\nu\beta\hbox{{\it 3}}\) in both the preimplantation embryo and outgrowing blastocyst. Also, integrin \(\alpha\nu\beta\hbox{{\it 3}}\) subunits were localized at the pericellular borders and cell–cell contact areas. The number of successful implantation sites of transferred HB-EGF-treated embryos in the uterus was increased when compared to number of implantation sites with non-treated controls.

Conclusions: HB-EGF may improve implantation by accelerating expression of integrin \(\alpha\nu\beta\hbox{{\it 3}}\) in peri-implantation mouse embryos.

J.J. Lim and D.R. Lee contributed equally to this work.