Effect of pentoxifylline in reducing oxidative stress-induced embryotoxicity
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Purpose: To 1) evaluate the embryotoxic effects of hydrogen peroxide on mouse embryo development and 2) examine if pentoxifylline can reverse hydrogen peroxide induced embryotoxicity.
Methods: Prospective in vitro study examining the effects of varying concentrations of hydrogen peroxide and pentoxifylline on the blastocyst development rate alone as well as in combination.
Results: A dose-dependent decrease in % BDR was seen with increasing concentrations of H2O2. High concentrations of hydrogen peroxide (> 60 μM) were embryotoxic. Pentoxifylline (500 μM) was able to reduce the embryotoxic effect of hydrogen peroxide. Percent blastocyst development rate increased from 44% in hydrogen peroxide alone to 85% in hydrogen peroxide and pentoxifylline coincubation.
Conclusions: Pentoxifylline may be beneficial in reducing hydrogen peroxide induced embryo damage and improve IVF outcome. Patients with endometriosis-associated infertility may benefit from the use of pentoxifylline without significantly affecting embryo development.
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- Effect of pentoxifylline in reducing oxidative stress-induced embryotoxicity
Journal of Assisted Reproduction and Genetics
Volume 22, Issue 11-12 , pp 415-417
- Cover Date
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- Online ISSN
- Kluwer Academic Publishers-Plenum Publishers
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- hydrogen peroxide
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- Author Affiliations
- 1. Center for Advanced Research in Human Reproduction, Infertility and Sexual Function, Department of Obstetrics-Gynecology and Glickman Urological Institute, Cleveland Clinic Foundation, Cleveland, Ohio
- 2. Desk A19.1, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, Ohio, 44195, USA