Animal Experimentation

Journal of Assisted Reproduction and Genetics

, Volume 22, Issue 11, pp 415-417

First online:

Effect of pentoxifylline in reducing oxidative stress-induced embryotoxicity

  • Xiaoyan ZhangAffiliated withCenter for Advanced Research in Human Reproduction, Infertility and Sexual Function, Department of Obstetrics-Gynecology and Glickman Urological Institute, Cleveland Clinic Foundation
  • , Rakesh K. SharmaAffiliated withCenter for Advanced Research in Human Reproduction, Infertility and Sexual Function, Department of Obstetrics-Gynecology and Glickman Urological Institute, Cleveland Clinic FoundationDesk A19.1, Cleveland Clinic Foundation Email author 
  • , Ashok AgarwalAffiliated withCenter for Advanced Research in Human Reproduction, Infertility and Sexual Function, Department of Obstetrics-Gynecology and Glickman Urological Institute, Cleveland Clinic Foundation
  • , Tommaso FalconeAffiliated withCenter for Advanced Research in Human Reproduction, Infertility and Sexual Function, Department of Obstetrics-Gynecology and Glickman Urological Institute, Cleveland Clinic Foundation

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Abstract

Purpose: To 1) evaluate the embryotoxic effects of hydrogen peroxide on mouse embryo development and 2) examine if pentoxifylline can reverse hydrogen peroxide induced embryotoxicity.

Methods: Prospective in vitro study examining the effects of varying concentrations of hydrogen peroxide and pentoxifylline on the blastocyst development rate alone as well as in combination.

Results: A dose-dependent decrease in % BDR was seen with increasing concentrations of H2O2. High concentrations of hydrogen peroxide (> 60 μM) were embryotoxic. Pentoxifylline (500 μM) was able to reduce the embryotoxic effect of hydrogen peroxide. Percent blastocyst development rate increased from 44% in hydrogen peroxide alone to 85% in hydrogen peroxide and pentoxifylline coincubation.

Conclusions: Pentoxifylline may be beneficial in reducing hydrogen peroxide induced embryo damage and improve IVF outcome. Patients with endometriosis-associated infertility may benefit from the use of pentoxifylline without significantly affecting embryo development.

Keywords

Embryotoxicity hydrogen peroxide pentoxifylline