Journal of Assisted Reproduction and Genetics

, Volume 22, Issue 11, pp 415–417

Effect of pentoxifylline in reducing oxidative stress-induced embryotoxicity

  • Xiaoyan Zhang
  • Rakesh K. Sharma
  • Ashok Agarwal
  • Tommaso Falcone
Animal Experimentation

DOI: 10.1007/s10815-005-7202-6

Cite this article as:
Zhang, X., Sharma, R.K., Agarwal, A. et al. J Assist Reprod Genet (2005) 22: 415. doi:10.1007/s10815-005-7202-6

Abstract

Purpose: To 1) evaluate the embryotoxic effects of hydrogen peroxide on mouse embryo development and 2) examine if pentoxifylline can reverse hydrogen peroxide induced embryotoxicity.

Methods: Prospective in vitro study examining the effects of varying concentrations of hydrogen peroxide and pentoxifylline on the blastocyst development rate alone as well as in combination.

Results: A dose-dependent decrease in % BDR was seen with increasing concentrations of H2O2. High concentrations of hydrogen peroxide (> 60 μM) were embryotoxic. Pentoxifylline (500 μM) was able to reduce the embryotoxic effect of hydrogen peroxide. Percent blastocyst development rate increased from 44% in hydrogen peroxide alone to 85% in hydrogen peroxide and pentoxifylline coincubation.

Conclusions: Pentoxifylline may be beneficial in reducing hydrogen peroxide induced embryo damage and improve IVF outcome. Patients with endometriosis-associated infertility may benefit from the use of pentoxifylline without significantly affecting embryo development.

Keywords

Embryotoxicityhydrogen peroxidepentoxifylline

Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Xiaoyan Zhang
    • 1
  • Rakesh K. Sharma
    • 1
    • 2
  • Ashok Agarwal
    • 1
  • Tommaso Falcone
    • 1
  1. 1.Center for Advanced Research in Human Reproduction, Infertility and Sexual Function, Department of Obstetrics-Gynecology and Glickman Urological InstituteCleveland Clinic FoundationCleveland
  2. 2.Desk A19.1Cleveland Clinic FoundationClevelandUSA