Journal of Applied Phycology

, Volume 27, Issue 1, pp 205–209

Microalgae biomass quantification by digital image processing and RGB color analysis

  • Mohammad H. Sarrafzadeh
  • Hyun-Joon La
  • Jae-Yon Lee
  • Dae-Hyun Cho
  • Sang-Yoon Shin
  • Woo-Jin Kim
  • Hee-Mock Oh
Article

DOI: 10.1007/s10811-014-0285-7

Cite this article as:
Sarrafzadeh, M.H., La, HJ., Lee, JY. et al. J Appl Phycol (2015) 27: 205. doi:10.1007/s10811-014-0285-7

Abstract

Digital image processing based on a red–green–blue (RGB) color analysis was applied to measure the cell concentration of three microalgae: Chlorella vulgaris, Botryococcus braunii, and Ettlia sp. The experiments were performed by using diluted and concentrated cultures of these microalgae to prepare different concentrations of dry cell weight (DCW). A charge-coupled device (CCD) camera was used to image the microalgae samples in a dark chamber homogenously illuminated from the bottom. The method showed to be a simple yet efficient technique for microalgae biomass estimation with an effective measurement range up to 3 g DCW L−1. Especially, the blue color value linearly decreased with DCW in this dynamic range of measurement in all the tested microalgae. The general correlation based on the conversion of RGB values to gray tones by application of a luminescence algorithm also showed similar patterns. The blue color value predicted the biomass concentrations of C. vulgaris, B. braunii, and Ettlia sp. with average errors of 13, 16, and 8 %, respectively, which were much lower than those of the gray tones conversion. Thus, the method presented in this study can be a base for the development of a more general method for microalgae biomass measurement.

Keywords

BiofuelsChlorophyllMicrobial biomass estimationPhotobioreactorRGB color model

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Mohammad H. Sarrafzadeh
    • 1
  • Hyun-Joon La
    • 2
  • Jae-Yon Lee
    • 2
  • Dae-Hyun Cho
    • 2
  • Sang-Yoon Shin
    • 2
    • 3
  • Woo-Jin Kim
    • 2
  • Hee-Mock Oh
    • 2
  1. 1.School of Chemical Engineering, College of EngineeringUniversity of TehranTehranIran
  2. 2.Environmental Biotechnology Research CenterKorea Research Institute of Bioscience and Biotechnology (KRIBB)DaejeonRepublic of Korea
  3. 3.Green Chemistry and Environmental BiotechnologyUniversity of Science and Technology (UST)DaejeonRepublic of Korea