, Volume 25, Issue 4, pp 1001-1007

Determination of oxidized scytonemin in Nostoc commune Vauch cultured on different conditions by high performance liquid chromatography coupled with triple quadrupole mass spectrometry

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Abstract

A facile method based on high performance liquid chromatography coupled with electrospray ionization tandem triple quadrupole mass spectrometry (HPLC-ESI-QqQ-MS) has been established to investigate the production of the oxidized scytonemin by Nostoc commune Vauch in different environmental conditions. In optimized HPLC-ESI-QqQ-MS conditions, the oxidized scytonemin can be effectively detected in selected reaction monitoring (SRM) mode. After regression, high linearity of the calibration curve was achieved with a correlation coefficient (r 2) at 0.99. The limit of detection and limit of quantification were 0.03 and 0.10 ng mL−1, respectively. The recovery of the oxidized scytonemin was at 76.90 %, and the relative standard deviations of inter- and intraday precisions were lower than 8.95 % (n = 4). Subsequently, the production of the oxidized scytonemin from N. commune has been investigated in different culture conditions. High culture temperature, strong illumination intensity, and light–dark cycle (12:12 h) were found to be good for producing the oxidized scytonemin by N. commune. In short, the HPLC-QqQ-SRM-MS method is a powerful tool for comprehensive analysis of the oxidized scytonemin from N. commune, owing to its excellent selectivity and sensitivity.