Journal of Applied Phycology

, Volume 24, Issue 3, pp 365–373

Screening for a low-cost Haematococcus pluvialis medium reveals an unexpected impact of a low N/P ratio on vegetative growth

  • Pierre Tocquin
  • Anthony Fratamico
  • Fabrice Franck
Article

DOI: 10.1007/s10811-011-9771-3

Cite this article as:
Tocquin, P., Fratamico, A. & Franck, F. J Appl Phycol (2012) 24: 365. doi:10.1007/s10811-011-9771-3

Abstract

The green alga Haematococcus pluvialis is the current best source of natural astaxanthin, a high-value carotenoid. Traditionally, the production process of astaxanthin by this algae is achieved by a two-stage system: during the first stage, vegetative “green” cells are produced and then converted, in the second stage, into cysts that accumulate astaxanthin. In this work, a medium screening strategy based on the mixing of a three-component hydroponic fertilizer was applied to identify a new formulation optimized for the vegetative stage. A maximal and high cell density of 2 × 106 cells mL−1 was obtained in a medium containing a high level of phosphate relative to nitrate, resulting in a N/P ratio much lower than commonly used media for H. pluvialis. In this medium, cells remained at the vegetative and motile stage during a prolonged period of time. Both high cell density culture and motile stage persistence was proved to be related to the N/P feature of this medium. We conclude that the macrozoid stage of H. pluvialis is favored under high-P and low-N supply and that low-cost hydroponic fertilizers can be successfully used for achieving high density cultures of vegetative cells of H. pluvialis.

Keywords

Haematococcus pluvialisCulture media optimizationPhosphate supplyNutrient-induced fluorescence transients

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Pierre Tocquin
    • 1
  • Anthony Fratamico
    • 1
  • Fabrice Franck
    • 1
  1. 1.Laboratory of Plant Biochemistry and Photobiology, Institute of Plant BiologyUniversity of LiègeLiègeBelgium