Journal of Applied Phycology

, Volume 17, Issue 6, pp 527–534

Antioxidant activity of different molecular weight sulfated polysaccharides from Ulva pertusa Kjellm (Chlorophyta)

  • Huimin Qi
  • Tingting Zhao
  • Quanbin Zhang
  • Zhien Li
  • Zengqin Zhao
  • Ronge Xing
Article

DOI: 10.1007/s10811-005-9003-9

Cite this article as:
Qi, H., Zhao, T., Zhang, Q. et al. J Appl Phycol (2005) 17: 527. doi:10.1007/s10811-005-9003-9

Abstract

Polysaccharides extracted from Ulva pertusa Kjellm (Chlorophyta) are a group of sulfated heteropolysaccharides, the ulvans. In this study, different molecular weight ulvans were prepared by H2O2 degradation and their antioxidant activities investigated including superoxide and hydroxyl radical scavenging activity, reducing power and metal chelating ability. The molecular weights of natural and degraded ulvans were 151.7, 64.5, 58.0, and 28.2 kDa, respectively, as determined by high performance gel permeation chromatography. Among the four samples, U3 (the lowest molecular weight sample) showed significant inhibitory effects on superoxide and hydroxyl radicals with IC50 values of 22.1 μ g mL−1 and 2.8 mg mL−1; its reducing power and metal chelating ability were also the strongest among the four samples. All the other samples also demonstrated strong activity against superoxide radicals. The results indicated that molecular weight had a significant effect on the antioxidant activity of ulvan with low molecular weight ulvan having stronger antioxidant activity.

Key words

antioxidant activity metal chelating ability molecular weight free radical scavenging effect reducing power ulvan 

Abbreviations:

U

polysaccharide extracted from U. pertusa (ulvan, Mw, 151.7 kDa)

U1

degraded ulvan of molecular weight 64.5 kDa

U2

degraded ulvan of molecular weight 58.0 kDa

U3

degraded ulvan of molecular weight 28.2 kDa

NBT

nitro blue tetrazolium

PMS

phenazine methosulfate

NADH

nicotinamide adenine dinucleotide-reduced

EDTA

ethylene diamine tetra-acetic acid

TCA

trichloroacetic acid

TBA

thiobarbituric acid

DR

deoxyribose

HPGPC

high performance gel permeation chromatography

GC

gas liquid chromatography

Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Huimin Qi
    • 1
    • 2
  • Tingting Zhao
    • 1
    • 2
  • Quanbin Zhang
    • 1
  • Zhien Li
    • 1
  • Zengqin Zhao
    • 1
    • 2
  • Ronge Xing
    • 1
    • 2
  1. 1.Institute of OceanologyChinese Academy of SciencesQingdaoP.R.China
  2. 2.Graduate School of the Chinese Academy of SciencesBeijingP.R. China

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