Journal of Applied Phycology

, Volume 17, Issue 6, pp 467–473

Genetic analysis of the position of meiosis in Porphyra haitanensis Chang et Zheng (Bangiales, Rhodophyta)

Article

DOI: 10.1007/s10811-005-2752-7

Cite this article as:
Yan, X., Li, L. & Aruga, Y. J Appl Phycol (2005) 17: 467. doi:10.1007/s10811-005-2752-7

Abstract

Crossing experiments were carried out between artificial pigmentation mutants and the wild type in Porphyra haitanensis Chang et Zheng to ascertain where meiosis occurs in its life history by confirming whether the color segregation and the color-sectored blades appear in F1 gametophytic blades developed from conchospores which are released from heterozygous conchocelis. Two red-type pigmentation mutants (R-10 and SPY-1) were used as the female parent. Their blades are red or red orange in color, thinner than the wild type and weak in elasticity, and have no denticles on their margins. The wild type (W) was used as the male parent; its blades are light brown in color, thick and good in elasticity, and have many marginal denticles. The F1 gametophytic blades developed from conchospores which were released from heterozygous conchocelis produced in the crosses of R-10(♀)×W(♂) and SPY-1(♀)×W(♂) showed two parental colors (R and W) and two new colors (R', lighter in color than R; W', wild-type-like color and redder than W). Linear segregation of colors occurred in the F1 blades, forming color-sectored blades with 2–4 sectors. In the color-sectored blades, R and R' sectors were thinner than W and W' sectors, and had weak elasticity and no denticles on their margins, whereas W and W' sectors were thick and had good elasticity and many marginal denticles. Of the F1 gametophytic blades, 95.2–96.7% were color-sectored and only 3.3–4.8% were unsectored. These results indicate that meiosis of P. haitanensis occurs during the first two cell divisions of a germinating conchospore, and thus it is considered that the initial four cells of a developing conchosporeling constitute a linear genetic tetrad leading to the formation of a color-sectored blade. The new colors of R' and W' were recombinant colors due to the chromosome recombination during the first cell division in meiosis. It is considered that color phenotypes of the two mutants used in this paper were result of two (or more) recessive mutations in different genes, and that they also have mutations concerned with blade thickness and formation of marginal denticles, which are linked with the color mutations.

Key words

color-sectored gametophytic blade cross experiment meiosis pigmentation mutant Porphyra haitanensis 

Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  1. 1.Key Laboratory of Germplasm Resources and Ecology in Aquaculture, Certificated by the Ministry of Agriculture, E-Institute of Shanghai Municipal Education CommissionShanghai Fisheries UniversityShanghaiPeoples' Republic of China
  2. 2.Tokyo University of AgricultureTokyoJapan