Journal of Applied Electrochemistry

, Volume 43, Issue 10, pp 985–994

l-Lactic acid biosensor based on multi-layered graphene

Authors

    • National Institute for Research and Development in Microtechnology (IMT-Bucharest)
  • Alexandru Cosmin Obreja
    • National Institute for Research and Development in Microtechnology (IMT-Bucharest)
  • Sandra A. V. Eremia
    • Centre of BioanalysisNational Institute of Research and Development for Biological Sciences
  • Adina Bragaru
    • National Institute for Research and Development in Microtechnology (IMT-Bucharest)
  • Adrian Dinescu
    • National Institute for Research and Development in Microtechnology (IMT-Bucharest)
  • Gabriel-Lucian Radu
    • Centre of BioanalysisNational Institute of Research and Development for Biological Sciences
Research Article

DOI: 10.1007/s10800-013-0594-6

Cite this article as:
Radoi, A., Obreja, A.C., Eremia, S.A.V. et al. J Appl Electrochem (2013) 43: 985. doi:10.1007/s10800-013-0594-6
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Abstract

Pristine graphene platelets and graphene oxide were used as electrode modifiers, aiming the investigation of their electrochemical efficacy towards β-nicotinamide adenine dinucleotide (NADH). The electrochemical detection of NADH is one of the most studied areas of bioelectroanalysis because of the ubiquity of NAD(P)H-based enzymatic reactions in nature. Commercially available graphene and laboratory prepared graphene oxide were used to modify glassy carbon electrodes and the behaviour of such modified electrodes against potassium ferricyanide (III) and NADH was reported. Relying on the graphene-modified transducer, l-lactic dehydrogenase (l-LDH) was successfully immobilised in a 1 % Nafion® membrane. The developed biosensor, working at +250 mV versus Ag/AgCl reference electrode, was used to assess l-lactic acid in four different types of yogurts, revealing an l-lactic acid concentration ranging between 0.3 and 0.6 %.

Keywords

GrapheneGraphene oxideNADHl-Lactic acidYogurtBiosensor

Abbreviations

NADH

β-nicotinamide adenine dinucleotide

NAD+

Oxidised form of β-nicotinamide adenine dinucleotide

G

Graphene

GO

Graphene oxide

GC

Modified glassy carbon

l-LDH

l-lactate dehydrogenase

Copyright information

© Springer Science+Business Media Dordrecht 2013