Article

InflammoPharmacology

, Volume 7, Issue 1, pp 47-62

PADMA-28, a traditional tibetan herbal preparation inhibits the respiratory burst in human neutrophils, the killing of epithelial cells by mixtures of oxidants and pro-inflammatory agonists and peroxidation of lipids

  • Isaac GinsburgAffiliated withDepartment of Oral Biology, Hebrew University-Hadassah School of Dental medicine
  • , Milu SadovnikAffiliated withDepartment of Oral Biology, Hebrew University-Hadassah School of Dental medicine
  • , Sarah SallonAffiliated withNatural Medicine Research Unit, Hadassah Hospital
  • , Ilana Milo-GoldzweigAffiliated withDepartment of Natural Products, School of Pharmacy, Faculty of Medicine
  • , Raphael MechoulamAffiliated withDepartment of Natural Products, School of Pharmacy, Faculty of Medicine
  • , Aviva BreuerAffiliated withDepartment of Natural Products, School of Pharmacy, Faculty of Medicine
  • , Douglas GibbsAffiliated withDepartment ofPathology, The University of Michigan
  • , James VaraniAffiliated withDepartment ofPathology, The University of Michigan
  • , Stanley RobertsAffiliated withDepartment of Chemistry, University of Liverpool
    • , Edward CleatorAffiliated withDepartment of Chemistry, University of Liverpool
    • , Neirmal SinghAffiliated withDepartment of Chemistry, University of Liverpool

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Abstract

Both aqueous and methanolic fractions derived from the Tibetan preparation PADMA-28 (a mixture of 22 plants) used as an anti-atherosclerotic agent, and which is non-cytolytic to a variety of mammalian cells, were found to strongly inhibit (1) the killing of epithelial cells in culture induced by ‘cocktails’ comprising oxidants, membrane perforating agents and proteinases; (2) the generation of luminol-dependent chemiluminescence in human neutrophils stimulated by opsonized bacteria; (3) the peroxidation of intralipid (a preparation rich in phopholipids) induced in the presence of copper; and (4) the activity of neutrophil elastase. It is proposed that PADMA-28 might prove beneficial for the prevention of cell damage induced by synergism among pro-inflammatory agonists which is central in the initiation of tissue destruction in inflammatory and infectious conditions.

Key words:

oxidative stress anti-oxidants cytolytic cocktail herbal preparation