Inflammation

, Volume 36, Issue 3, pp 670–679

Epidermal Growth Factor Promotes Proliferation and Improves Restoration After Intestinal Ischemia–Reperfusion Injury in Rats

  • Yanxia Geng
  • Jieshou Li
  • Fan Wang
  • Qiurong Li
  • Xing Wang
  • Liqun Sun
  • Weiqin Li
Article

DOI: 10.1007/s10753-012-9591-x

Cite this article as:
Geng, Y., Li, J., Wang, F. et al. Inflammation (2013) 36: 670. doi:10.1007/s10753-012-9591-x

Abstract

Epidermal growth factor (EGF) is an attractive and promising therapeutic application for intestinal disorders. The current study examined its influence on proliferation and restoration after ischemia–reperfusion (I/R) injury in rat small intestine. Six groups were performed: sham operation (Con); ischemia for 30 min with subsequent reperfusion for 30 min (I/R); I/R injured with 500 μg/kg EGF injected 5 min before ischemia (Pre-l); I/R injured with 50 μg/kg EGF injected 5 min before ischemia (Pre-s); I/R injured with 500 μg/kg EGF injected 5 min after reperfusion (Post-l); and I/R injured with 50 μg/kg EGF injected 5 min after reperfusion (Post-s). Intestinal histological damage, crypt cell proliferation degree, mucosal permeability, tight junction proteins expression, and levels of inflammation factors were studied for each group. Compared with the I/R group, administration of EGF in the Pre-l, Pre-s, and Post-l groups all presented a significant proliferation effect. The levels of FD4, IL-6, and TNF-α were dramatically decreased in all EGF-treated groups. Histological destruction was improved and TJs recovery was notably accelerated in all EGF-treated groups except the Post-s group. d-lactate concentration was only diminished in the Pre-l group. These results suggest that mucosally applied EGF can promote intestinal proliferation and improve restoration after I/R injury. EGF intraluminal administration is an effective treatment against intestinal I/R injury.

KEY WORDS

epidermal growth factorischemia–reperfusionproliferationintestinal barrier functionrecovery

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Yanxia Geng
    • 1
  • Jieshou Li
    • 2
  • Fan Wang
    • 3
  • Qiurong Li
    • 2
  • Xing Wang
    • 4
  • Liqun Sun
    • 5
  • Weiqin Li
    • 2
  1. 1.Department of Intensive Care UnitJiangsu Province Chinese Medicine HospitalNanjingChina
  2. 2.Department of SurgeryJinling HospitalNanjingChina
  3. 3.The Affiliated Drum Tower Hospital of Medical School of Nanjing UniversityNanjingChina
  4. 4.Department of Intensive Care UnitJiangsu Province Chinese Medicine HospitalNanjingChina
  5. 5.Department of Intensive Care UnitThe Second Affiliated Hospital of Nanjing Medical UniversityNanjingChina