Inflammation

, Volume 36, Issue 3, pp 670–679

Epidermal Growth Factor Promotes Proliferation and Improves Restoration After Intestinal Ischemia–Reperfusion Injury in Rats

Authors

  • Yanxia Geng
    • Department of Intensive Care UnitJiangsu Province Chinese Medicine Hospital
    • Department of SurgeryJinling Hospital
  • Fan Wang
    • The Affiliated Drum Tower Hospital of Medical School of Nanjing University
  • Qiurong Li
    • Department of SurgeryJinling Hospital
  • Xing Wang
    • Department of Intensive Care UnitJiangsu Province Chinese Medicine Hospital
  • Liqun Sun
    • Department of Intensive Care UnitThe Second Affiliated Hospital of Nanjing Medical University
  • Weiqin Li
    • Department of SurgeryJinling Hospital
Article

DOI: 10.1007/s10753-012-9591-x

Cite this article as:
Geng, Y., Li, J., Wang, F. et al. Inflammation (2013) 36: 670. doi:10.1007/s10753-012-9591-x

Abstract

Epidermal growth factor (EGF) is an attractive and promising therapeutic application for intestinal disorders. The current study examined its influence on proliferation and restoration after ischemia–reperfusion (I/R) injury in rat small intestine. Six groups were performed: sham operation (Con); ischemia for 30 min with subsequent reperfusion for 30 min (I/R); I/R injured with 500 μg/kg EGF injected 5 min before ischemia (Pre-l); I/R injured with 50 μg/kg EGF injected 5 min before ischemia (Pre-s); I/R injured with 500 μg/kg EGF injected 5 min after reperfusion (Post-l); and I/R injured with 50 μg/kg EGF injected 5 min after reperfusion (Post-s). Intestinal histological damage, crypt cell proliferation degree, mucosal permeability, tight junction proteins expression, and levels of inflammation factors were studied for each group. Compared with the I/R group, administration of EGF in the Pre-l, Pre-s, and Post-l groups all presented a significant proliferation effect. The levels of FD4, IL-6, and TNF-α were dramatically decreased in all EGF-treated groups. Histological destruction was improved and TJs recovery was notably accelerated in all EGF-treated groups except the Post-s group. d-lactate concentration was only diminished in the Pre-l group. These results suggest that mucosally applied EGF can promote intestinal proliferation and improve restoration after I/R injury. EGF intraluminal administration is an effective treatment against intestinal I/R injury.

KEY WORDS

epidermal growth factor ischemia–reperfusion proliferation intestinal barrier function recovery

Copyright information

© Springer Science+Business Media New York 2013