Inflammation

, Volume 36, Issue 3, pp 616–624

Zinc Sulfate Inhibited Inflammation of Der p2-Induced Airway Smooth Muscle Cells by Suppressing ERK1/2 and NF-κB Phosphorylation

Authors

  • Chia-Ju Shih
    • Department of Medical Research, Kuang Tien General Hospital
    • Department of Nutrition & Institute of BioMedical NutritionHungkuang University
Article

DOI: 10.1007/s10753-012-9583-x

Cite this article as:
Shih, C. & Chiou, Y. Inflammation (2013) 36: 616. doi:10.1007/s10753-012-9583-x

Abstract

Inflammation of airway smooth muscle cells (ASMCs) is believed to be important in causing airway hyperresponsiveness. However, zinc has been reported to be implicated in many kinds of cell inflammation. Little is known about the effect of zinc treatment on Der p2 (group II Dermatophagoides pteronyssinus)-induced inflammation from ASMCs. This study investigated effects and mechanisms of zinc in Der p2-treated ASMCs. Der p2-treated primary ASMCs were cultured with various concentrations of zinc sulfate (ZnSO4) 6 μM, 12 μM, 24 μM, and 96 μM. The proteins and mRNAs of cytokines in ASMCs were examined by ELISA and real-time PCR. Intracellular zinc was stained with Zinquin fluorescence. The cell signaling protein expression was detected by Western blot. Der p2 was used to induce interleukin (IL)-6, IL-8, IL-1, and monocyte chemotactic protein-1 production of ASMCs. However, we found that 24 μM ZnSO4 reduced these inflammatory mediators production of Der p2-treated primary ASMCs. Der p2-induced extracellular signal-regulated kinases (ERK) and nuclear factor-kappa B (NF-κB) phosphorylation were suppressed by supplementation of 24 μM ZnSO4. Zinc is an anti-inflammatory agent that reduces inflammation of Der p2-treated ASMCs through the suppression of the ERK and NF-κB pathway. The results may be helpful for the development of effective treatments.

KEY WORDS

zincasthmaairway smooth muscle cellsDer p2inflammation

ABBREVIATIONS

ASMCs

Airway smooth muscle cells

AHR

Airway hyperresponsiveness

Der p2

Group II Dermatophagoides pteronyssinus

ERK

Extracellular signal-regulated kinases

NF-κB

Nuclear factor-kappa B

HBSS

Hanks’ balanced salt solution

DMEM

Dulbecco’s modified Eagle’s medium

FBS

Fetal bovine serum

IL

Interleukin

MCP

Monocyte chemotactic protein

HDM

House dust mite

BSA

Bovine serum albumin

FBS

Fetal bovine serum

SDS

Sodium dodecyl sulfate

Copyright information

© Springer Science+Business Media New York 2012