Article

Inflammation

, Volume 35, Issue 6, pp 1860-1866

First online:

Protective Effect of Magnolol on Lipopolysaccharide-Induced Acute Lung Injury in Mice

  • Yun Feng NiAffiliated withDepartment of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University
  • , Tao JiangAffiliated withDepartment of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University
  • , Qing Shu ChengAffiliated withDepartment of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University
  • , Zhong Ping GuAffiliated withDepartment of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University
  • , Yi Fang ZhuAffiliated withDepartment of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University
  • , Zhi Pei ZhangAffiliated withDepartment of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University
  • , Jian WangAffiliated withDepartment of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University
  • , Xiao Long YanAffiliated withDepartment of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University
  • , Wu Ping WangAffiliated withDepartment of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University
    • , Chang Kang KeAffiliated withDepartment of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University
    • , Yong HanAffiliated withDepartment of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University Email author 
    • , Xiao Fei LiAffiliated withDepartment of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University Email author 

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Abstract

Magnolol, a tradition Chinese herb, displays an array of activities including antifungal, antibacterial, and antioxidant effects. To investigate the protective effect of magnolol on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice. ALI was induced in mice by intratracheal instillation of LPS (1 mg/kg). The mice received intratracheal instillation of magnolol (5 μg/kg) 30 min before LPS administration. Pulmonary histological changes were evaluated by hematoxylin-eosin stain and lung wet/dry weight ratios were observed. Concentrations of tumor necrosis factor (TNF)-α and interleukin (IL)-1β, and myeloperoxidase (MPO) activity were measured by enzyme-linked immunosorbent assay. Expression of cyclooxygenase (COX)-2 in lung tissues was determined by Western blot analysis. Magnolol pretreatment significantly attenuated the severity of lung injury and inhibited the production of TNF-α and IL-1β in mice with ALI. After LPS administration, the lung wet/dry weight ratios, as an index of lung edema, and MPO activity were also markedly reduced by magnolol pretreatment. The expression of COX-2 was significantly suppressed by magnolol pretreatment. Magnolol potently protected against LPS-induced ALI and the protective effects of magnolol may attribute partly to the suppression of COX-2 expression.

KEY WORDS

lipopolysaccharide acute lung injury magnolol cyclooxygenase-2 tumor necrosis factor-α interleukin-1β