Article

Inflammation

, Volume 35, Issue 5, pp 1659-1668

First online:

Sulfated Derivative of 20(S)-Ginsenoside Rh2 Inhibits Inflammatory Cytokines Through MAPKs and NF-kappa B Pathways in LPS-Induced RAW264.7 Macrophages

  • Wen-Yan BiAffiliated withDepartment of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Jilin University
  • , Ben-Dong FuAffiliated withDepartment of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Jilin University
  • , Hai-Qing ShenAffiliated withDepartment of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Jilin University
  • , Qian WeiAffiliated withDepartment of Cardiology, Affiliated Hospital, Changchun University of Chinese Medicine
  • , Cui ZhangAffiliated withDepartment of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Jilin University
  • , Zhou SongAffiliated withDepartment of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Jilin University
  • , Qian-Qian QinAffiliated withDepartment of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Jilin University
  • , Hui-Ping LiAffiliated withDepartment of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Jilin University
  • , Shuang LvAffiliated withDepartment of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Jilin University
    • , Shuai-Cheng WuAffiliated withDepartment of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Jilin University
    • , Peng-Fei YiAffiliated withDepartment of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Jilin University
    • , Xu-Bin WeiAffiliated withDepartment of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Jilin University Email author 

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

In the previous study, we found that sulfated derivative B2 of ginsenoside Rh2 (Rh2-B2) has greater anti-inflammatory effects than 20(S)-ginsenoside Rh2. However, the anti-inflammatory mechanism of Rh2-B2 remains unclear. We therefore assessed the effects of Rh2-B2 on inflammatory cytokines in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. We found that Rh2-B2 (1–5 mg/L) significantly inhibited tumor necrosis factor alpha, interleukin (IL)-6, IL-1β, and increased IL-10 production from protein and mRNA levels. Furthermore, Rh2-B2 significantly inhibited the phosphorylation of p38 and c-Jun N-terminal kinase as well as decreased p65 nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) translocation into the nucleus by nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha degradation. The present results indicate that Rh2-B2 inhibits the production of inflammatory cytokines induced by LPS through blocking mitogen-activated protein kinases and NF-κB signaling pathways.

KEY WORDS

ginsenoside Rh2 derivative inflammation signaling pathway