Inflammation

, Volume 35, Issue 5, pp 1659–1668

Sulfated Derivative of 20(S)-Ginsenoside Rh2 Inhibits Inflammatory Cytokines Through MAPKs and NF-kappa B Pathways in LPS-Induced RAW264.7 Macrophages

  • Wen-Yan Bi
  • Ben-Dong Fu
  • Hai-Qing Shen
  • Qian Wei
  • Cui Zhang
  • Zhou Song
  • Qian-Qian Qin
  • Hui-Ping Li
  • Shuang Lv
  • Shuai-Cheng Wu
  • Peng-Fei Yi
  • Xu-Bin Wei
Article

DOI: 10.1007/s10753-012-9482-1

Cite this article as:
Bi, WY., Fu, BD., Shen, HQ. et al. Inflammation (2012) 35: 1659. doi:10.1007/s10753-012-9482-1

Abstract

In the previous study, we found that sulfated derivative B2 of ginsenoside Rh2 (Rh2-B2) has greater anti-inflammatory effects than 20(S)-ginsenoside Rh2. However, the anti-inflammatory mechanism of Rh2-B2 remains unclear. We therefore assessed the effects of Rh2-B2 on inflammatory cytokines in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. We found that Rh2-B2 (1–5 mg/L) significantly inhibited tumor necrosis factor alpha, interleukin (IL)-6, IL-1β, and increased IL-10 production from protein and mRNA levels. Furthermore, Rh2-B2 significantly inhibited the phosphorylation of p38 and c-Jun N-terminal kinase as well as decreased p65 nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) translocation into the nucleus by nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha degradation. The present results indicate that Rh2-B2 inhibits the production of inflammatory cytokines induced by LPS through blocking mitogen-activated protein kinases and NF-κB signaling pathways.

KEY WORDS

ginsenoside Rh2derivativeinflammationsignaling pathway

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Wen-Yan Bi
    • 1
  • Ben-Dong Fu
    • 1
  • Hai-Qing Shen
    • 1
  • Qian Wei
    • 2
  • Cui Zhang
    • 1
  • Zhou Song
    • 1
  • Qian-Qian Qin
    • 1
  • Hui-Ping Li
    • 1
  • Shuang Lv
    • 1
  • Shuai-Cheng Wu
    • 1
  • Peng-Fei Yi
    • 1
  • Xu-Bin Wei
    • 1
  1. 1.Department of Clinical Veterinary Medicine, College of Animal Science and Veterinary MedicineJilin UniversityChangchunChina
  2. 2.Department of Cardiology, Affiliated HospitalChangchun University of Chinese MedicineChangchunChina