Inflammation

, Volume 35, Issue 2, pp 671–683

Neutrophilic Inflammatory Response and Oxidative Stress in Premenopausal Women Chronically Exposed to Indoor Air Pollution from Biomass Burning

  • Anirban Banerjee
  • Nandan Kumar Mondal
  • Debangshu Das
  • Manas Ranjan Ray
Article

DOI: 10.1007/s10753-011-9360-2

Cite this article as:
Banerjee, A., Mondal, N.K., Das, D. et al. Inflammation (2012) 35: 671. doi:10.1007/s10753-011-9360-2

Abstact

The possibility of inflammation and neutrophil activation in response to indoor air pollution (IAP) from biomass fuel use has been investigated. For this, 142 premenopausal, never-smoking women (median age, 34 years) who cook exclusively with biomass (wood, dung, crop wastes) and 126 age-matched control women who cook with cleaner fuel liquefied petroleum gas (LPG) were enrolled. The neutrophil count in blood and sputum was significantly higher (p < 0.05) in biomass users than the control group. Flow cytometric analysis revealed marked increase in the surface expression of CD35 (complement receptor-1), CD16 (FCγ receptor III), and β2 Mac-1 integrin (CD11b/CD18) on circulating neutrophils of biomass users. Besides, enzyme-linked immunosorbent assay showed that they had 72%, 67%, and 54% higher plasma levels of the proinflammatory cytokines tumor necrosis factor-alpha, interleukin-6, and interleukin-12, respectively, and doubled neutrophil chemoattractant interleukin-8. Immunocytochemical study revealed significantly higher percentage of airway neutrophils expressing inducible nitric oxide synthase, while the serum level of nitric oxide was doubled in women who cooked with biomass. Spectrophotometric analysis documented higher myeloperoxidase activity in circulating neutrophils of biomass users, suggesting neutrophil activation. Flow cytometry showed excess generation of reactive oxygen species (ROS) by leukocytes of biomass-using women, whereas their erythrocytes contained a depleted level of antioxidant enzyme superoxide dismutase (SOD). Indoor air of biomass-using households had two to four times more particulate matter with diameters of <10 μm (PM10) and <2.5 μm (PM2.5) as measured by real-time laser photometer. After controlling potential confounders, rise in proinflammatory mediators among biomass users were positively associated with PM10 and PM2.5 in indoor air, suggesting a close relationship between IAP and neutrophil activation. Besides, the levels of neutrophil activation and inflammation markers were positively associated with generation of ROS and negatively with SOD, indicating a role of oxidative stress in mediating neutrophilic inflammatory response following chronic inhalation of biomass smoke.

KEY WORDS

biomass fuelneutrophilinflammationoxidative stresspremenopausal womenIndia

ABBREVIATIONS

ACD

acid citrate dextrose

BMF

biomass fuel

BSA

bovine serum albumin

DCF-DA

dichlorofluorescein diacetate

EDTA

ethylenediaminetetraacetic acid

ELISA

enzyme-linked immunosorbent assay

FACS

fluorescence-activated cell sorter

FITC

fluorescein isothiocyanate

HRP

horseradish peroxidase

IAP

indoor air pollution

ICC

immunocytochemistry

IL

interleukin

iNOS

inducible nitric oxide synthase

LPG

liquefied petroleum gas

MFI

mean fluorescence intensity

MPO

myeloperoxidase

NO

nitric oxide

PAP

Papanicolaou

PBS

phosphate-buffered saline

PE

phycoerythrin

PM

particulate matter

ROS

reactive oxygen species

SOD

superoxide dismutase

TNF

tumor necrosis factor

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Anirban Banerjee
    • 1
  • Nandan Kumar Mondal
    • 1
  • Debangshu Das
    • 1
  • Manas Ranjan Ray
    • 1
  1. 1.Department of Experimental HematologyChittaranjan National Cancer InstituteKolkataIndia