Inflammation

, Volume 35, Issue 1, pp 89–97

Inhibitory Effects of Prior Low-dose X-irradiation on Cold-induced Brain Injury in Mouse

  • Masaaki Yoshimoto
  • Takahiro Kataoka
  • Teruaki Toyota
  • Takehito Taguchi
  • Kiyonori Yamaoka
Article

DOI: 10.1007/s10753-011-9293-9

Cite this article as:
Yoshimoto, M., Kataoka, T., Toyota, T. et al. Inflammation (2012) 35: 89. doi:10.1007/s10753-011-9293-9

Abstract

We examined the inhibitory effects of low-dose X-irradiation on mouse brain tissue with cold-induced injury by comparing tissue samples from three groups of mice: control, sham-irradiated cold-exposed, and X-ray-irradiated (0.5 Gy) cold-exposed mice. The water content in brain increased significantly in the sham-irradiated group following the cold-induced injury relative to the control group. However, water content in brain tissue from the X-ray-irradiated group was significantly lower than that from the sham-irradiated group. Levels of antioxidants, such as superoxide dismutase and glutathione, in brain tissue from the X-ray-irradiated group were higher than those from the sham-irradiated group. Moreover, the cold injury-induced cell death, particularly apoptosis, while low-dose irradiation inhibited cell death, especially among glial cells, but not numeral cells. These findings suggest that prior low-dose X-irradiation activated antioxidant function and inhibited cold-induced brain injury.

KEY WORDS

cold injurybrain edema0.5 Gy irradiationantioxidative function

Abbreviations

BBB

Blood–brain barrier

ROS

Reactive oxygen species

SOD

Superoxide dismutase

GPx

Glutathione peroxidase

GR

Glutathione reductase

Total GSH

Total glutathione

EDTA

Ethylenediaminetetraacetic acid

NBT

Nitroblue tetrazolium

H2O2

Hydrogen peroxide

MDA

Malondialdehyde

HE

Hematoxylin–eosin

KB

Kluver–Barrera

TUNEL

Terminal dUTP in situ nick-end labeling

PBS

Phosphate-buffered saline

DAB

3,39-diaminobenzidine

SEM

Standard error of the mean

MCA

Middle cerebral artery

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Masaaki Yoshimoto
    • 1
  • Takahiro Kataoka
    • 1
  • Teruaki Toyota
    • 1
  • Takehito Taguchi
    • 1
  • Kiyonori Yamaoka
    • 1
  1. 1.Graduate School of Health SciencesOkayama UniversityOkayama-shiJapan