Inflammation

, Volume 33, Issue 2, pp 126–136

Schisantherin A Exhibits Anti-inflammatory Properties by Down-Regulating NF-κB and MAPK Signaling Pathways in Lipopolysaccharide-Treated RAW 264.7 Cells

Authors

  • Xinxin Ci
    • Department of Veterinary Pharmacology, College of Animal Science and Veterinary MedicineJilin University
  • Rong Ren
    • The College of Life Sciences and TechnologyKunming University of Science and Technology
  • Kan Xu
    • Department of Veterinary Pharmacology, College of Animal Science and Veterinary MedicineJilin University
  • Hongyu Li
    • Department of Veterinary Pharmacology, College of Animal Science and Veterinary MedicineJilin University
  • Qinlei Yu
    • Jilin Provincial Center for Animal Disease Control and Prevention
  • Yu Song
    • Department of Veterinary Pharmacology, College of Animal Science and Veterinary MedicineJilin University
  • Dacheng Wang
    • Department of Veterinary Pharmacology, College of Animal Science and Veterinary MedicineJilin University
  • Rongtao Li
    • The College of Life Sciences and TechnologyKunming University of Science and Technology
    • Department of Veterinary Pharmacology, College of Animal Science and Veterinary MedicineJilin University
Article

DOI: 10.1007/s10753-009-9166-7

Cite this article as:
Ci, X., Ren, R., Xu, K. et al. Inflammation (2010) 33: 126. doi:10.1007/s10753-009-9166-7

Abstract

Schisantherin A, a dibenzocyclooctadiene lignan isolated from the fruit of Schisandra sphenanthera, has been used as an antitussive, tonic, and sedative agent under the name of Wuweizi in Chinese traditional medicine. In the present study, we carry out a screening program to identify the anti-inflammatory potentials of schisantherin A. We found that schisantherin A reduced lipopolysaccharide (LPS (1 mg/L))-induced levels of TNF-α, IL-6, NO, and PGE2 (p < 0.01 or p < 0.05), and also reduced levels of iNOS and COX-2 in RAW 264.7 macrophages in a concentration-dependent manner. We further investigated signal transduction mechanisms to determine how schisantherin A affects. RAW264.7 cells were pretreated with 0.5, 2.5, or 25 mg/L of schisantherin A 1 h prior to treatment with 1 mg/L of LPS. Thirty minutes later, cells were harvested and mitogen activated protein kinases (MAPKs) activation and IκBα was measured by Western blot. Alternatively, cells were fixed and nuclear factor-κB (NF-κB) activation was measured using immunocytochemical analysis. Signal transduction studies showed that schisantherin A significantly inhibited extracellular signal-regulated kinase (ERK), p38, and c-jun NH2-terminal kinase (JNK) phosphorylation protein expression. Schisantherin A also inhibited p65-NF-κB translocation into the nucleus by IκBα degradation. By using specific inhibitors of ERK, JNK and p38, we found that schisantherin A may inhibit TNF-α mostly through ERK pathway. Therefore, schisantherin A may inhibit LPS-induced production of inflammatory cytokines by blocking NF-κB and MAPKs signaling in RAW264.7 cells.

KEY WORDS

schisantherin AcytokinesNOPGE2iNOSCOX-2NF-κB and MAPKs

Copyright information

© Springer Science+Business Media, LLC 2009