Inflammation

, Volume 31, Issue 2, pp 133–140

Japanese Encephalitis Virus Envelope Protein Mitigates TNF-α mRNA Expression in RAW264.7 Cells

Authors

  • Tsai-Ching Hsu
    • Institute of ImmunologyChung Shan Medical University
  • Ji-Qiang Gao
    • Institute of Biochemistry and BiotechnologyChung Shan Medical University
  • Ko-Hsiu Lu
    • School of MedicineChung Shan Medical University
    • Department of Orthopaedic SurgeryChung Shan Medical University Hospital
  • Chang-Hai Tsai
    • Department of Healthcare AdministrationAsia University
  • Chih-Yang Huang
    • Graduate Institute of Chinese Medical ScienceChina Medical University
    • Institute of Basic Medical ScienceChina Medical University
    • Department of Health and Nutrition BiotechnologyAsia University
    • Institute of Biochemistry and BiotechnologyChung Shan Medical University
Article

DOI: 10.1007/s10753-008-9058-2

Cite this article as:
Hsu, T., Gao, J., Lu, K. et al. Inflammation (2008) 31: 133. doi:10.1007/s10753-008-9058-2

Abstract

Japanese encephalitis virus (JEV) is known as an important mosquito-borne human pathogen that causes Japanese encephalitis and may lead to lethal effect. Since monocyte has been demonstrated to play transmissible role for JEV, rare study is reported to clarify the effect of JEV envelope (JEVE) protein on monocyte. This study intends to investigate the effects of JEVE protein inside monocyte. Notably, significant decreased expression of tumour necrosis factor (TNF)-α mRNA in RAW264.7 cells transfected with pEGFP-JEVE was observed as compared to those cells transfected with pEGFP. Increased p21Waf1/Cip1 protein was observed in both pEGFP and pEGFP-JEVE transfected RAW264.7 cells. However, increased p53 protein was only detected in pEGFP-transfected cells but not pEGFP-JEVE transfected cells as well as the result that no increased expression of nuclear factor-kB was observed in pEGFP-JEVE transfected cells. These experimental results indicate the effects of JEVE protein in alleviating TNF-α mRNA expression that is associated with the increased p53-independent p21Waf1/Cip1 expression and provide an explanation in the role of JEV transmission through monocyte.

Key words

Japanese encephalitis virusmonocyteenvelope proteinTNF-αp21Waf1/Cip1

Abbreviations

JEV

Japanese encephalitis virus

TNF

tumour necrosis factor

NF-kB

nuclear factor-kB

Copyright information

© Springer Science+Business Media, LLC 2008