, Volume 31, Issue 2, pp 133-140

First online:

Japanese Encephalitis Virus Envelope Protein Mitigates TNF-α mRNA Expression in RAW264.7 Cells

  • Tsai-Ching HsuAffiliated withInstitute of Immunology, Chung Shan Medical University
  • , Ji-Qiang GaoAffiliated withInstitute of Biochemistry and Biotechnology, Chung Shan Medical University
  • , Ko-Hsiu LuAffiliated withSchool of Medicine, Chung Shan Medical UniversityDepartment of Orthopaedic Surgery, Chung Shan Medical University Hospital
  • , Chang-Hai TsaiAffiliated withDepartment of Healthcare Administration, Asia University
  • , Chih-Yang HuangAffiliated withGraduate Institute of Chinese Medical Science, China Medical UniversityInstitute of Basic Medical Science, China Medical UniversityDepartment of Health and Nutrition Biotechnology, Asia University
  • , Bor-Show TzangAffiliated withInstitute of Biochemistry and Biotechnology, Chung Shan Medical University Email author 

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Japanese encephalitis virus (JEV) is known as an important mosquito-borne human pathogen that causes Japanese encephalitis and may lead to lethal effect. Since monocyte has been demonstrated to play transmissible role for JEV, rare study is reported to clarify the effect of JEV envelope (JEVE) protein on monocyte. This study intends to investigate the effects of JEVE protein inside monocyte. Notably, significant decreased expression of tumour necrosis factor (TNF)-α mRNA in RAW264.7 cells transfected with pEGFP-JEVE was observed as compared to those cells transfected with pEGFP. Increased p21Waf1/Cip1 protein was observed in both pEGFP and pEGFP-JEVE transfected RAW264.7 cells. However, increased p53 protein was only detected in pEGFP-transfected cells but not pEGFP-JEVE transfected cells as well as the result that no increased expression of nuclear factor-kB was observed in pEGFP-JEVE transfected cells. These experimental results indicate the effects of JEVE protein in alleviating TNF-α mRNA expression that is associated with the increased p53-independent p21Waf1/Cip1 expression and provide an explanation in the role of JEV transmission through monocyte.

Key words

Japanese encephalitis virus monocyte envelope protein TNF-α p21Waf1/Cip1