Journal of Molecular Histology

, Volume 44, Issue 5, pp 565–573

Expression and redistribution of β-catenin in the cardiac myocytes of left ventricle of spontaneously hypertensive rat

Authors

  • Qiaoli Zheng
    • Department of PathologySun Yat-sen University the Fifth Affiliated Hospital
  • Ping Chen
    • Department of PathologyJiangmen Central Hospital
  • Zeqing Xu
    • Department of PathologySun Yat-sen University the Fifth Affiliated Hospital
  • Faqian Li
    • Department of Pathology and Laboratory MedicineUniversity of Rochester Medical Center
    • Department of PathologySun Yat-sen University the Fifth Affiliated Hospital
Original Paper

DOI: 10.1007/s10735-013-9507-6

Cite this article as:
Zheng, Q., Chen, P., Xu, Z. et al. J Mol Hist (2013) 44: 565. doi:10.1007/s10735-013-9507-6

Abstract

Beta-catenin is not only an adhering junction protein, but also the central player of the canonical Wnt signalling pathway. In order to investigate the roles of β-catenin in the mechanism of myocardial hypertrophy, we determined the expression and distribution of β-catenin in the cardiomyocytes of spontaneously hypertensive heart failure (SHHF) rats and age-matched Wistar-Kyoto (WKY) rats. We identified the reducing of β-catenin expression in the membrane protein fraction but increasing in the nuclear protein in the 6 and 12 month-old SHHF rats as compared with the age-matched WKY rats by Western blotting. Immunolabeling of β-catenin colocalized with cadherin at the intercalated disc sites and showed nuclear accumulation in myocytes of SHHF rats. We also revealed that the association between glycogen synthase kinase-3β and β-catenin had weakened in the 6 month-old SHHF rats as compared with the age-matched WKY rats by immunoprecipitation. These findings suggested that nuclear translocation of β-catenin might play important roles in regulating signal transduction in the decompensated hypertrophy stage.

Keywords

Cardiac hypertrophyBeta-cateninCadherinGlycogen synthase kinase-3β (GSK3β)Signal transduction

Copyright information

© Springer Science+Business Media Dordrecht 2013