Journal of Molecular Histology

, Volume 44, Issue 1, pp 27–36

Increased expression of Gem after rat sciatic nerve injury

Authors

  • Youhua Wang
    • Department of OrthopaedicsAffiliated Hospital of Nantong University
  • Xinghai Cheng
    • Department of OrthopaedicsAffiliated Hospital of Nantong University
  • Zhengming Zhou
    • Department of OrthopaedicsAffiliated Hospital of Nantong University
  • Hao Wu
    • Department of OrthopaedicsAffiliated Hospital of Nantong University
  • Long Long
    • Department of OrthopaedicsAffiliated Hospital of Nantong University
    • The Jiangsu Province Key Laboratory of NeuroregenerationNantong University
    • Department of OrthopaedicsAffiliated Hospital of Nantong University
Original Paper

DOI: 10.1007/s10735-012-9459-2

Cite this article as:
Wang, Y., Cheng, X., Zhou, Z. et al. J Mol Hist (2013) 44: 27. doi:10.1007/s10735-012-9459-2

Abstract

Gem belongs to the Rad/Gem/Kir subfamily of Ras-related GTPases, whose expression is induced in several cell types upon activation by extracellular stimuli. Two functions of Gem have been demonstrated, including regulation of voltage-gated calcium channel activity and inhibition of Rho kinase-mediated cytoskeletal reorganization, such as stress fiber formation and neurite retraction. Because of the essential relationship between actin reorganization and peripheral nerve regeneration, we investigated the spatiotemporal expression of Gem in a rat sciatic nerve crush (SNC) model. After never injury, we observed that Gem had a significant up-regulation from 1 day, peaked at day 5 and then gradually decreased to the normal level. At its peak expression, Gem expressed mainly in Schwann cells (SCs) and macrophages of the distal sciatic nerve segment, but had few colocalization in axons. In addition, the peak expression of Gem was in parallel with PCNA, and numerous SCs expressing Gem were PCNA positive. Thus, all of our findings suggested that Gem may be involved in the pathophysiology of sciatic nerve after SNC.

Keywords

GemSciatic nerve crushSchwann cellsMacrophagesNeurogenesisRat

Abbreviations

SNC

Sciatic nerve crush

SCs

Schwann cells

ROK

Rho kinase

CK2

Casein kinase 2

ERK

Extracellular signal-regulated kinase

PAGE

Polyacrylamide gel electrophoresis

ECL

Enhanced chemiluminescence system

BSA

Bovine serum albumin

DAB

Diaminobenzidin

PCNA

Proliferating cell nuclear antigen

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

Copyright information

© Springer Science+Business Media Dordrecht 2012