Original Paper

Journal of Molecular Histology

, Volume 41, Issue 6, pp 379-386

Tissue distribution and expression of paraoxonases and chemokines in mouse: the ubiquitous and joint localisation suggest a systemic and coordinated role

  • Fernando Rodríguez-SanabriaAffiliated withCentre de Recerca Biomèdica. Hospital Universitari de Sant Joan. Institut d´Investigació Sanitària Pere Virgili (IISPV), Universitat Rovira i Virgili
  • , Anna RullAffiliated withCentre de Recerca Biomèdica. Hospital Universitari de Sant Joan. Institut d´Investigació Sanitària Pere Virgili (IISPV), Universitat Rovira i Virgili
  • , Raúl Beltrán-DebónAffiliated withCentre de Recerca Biomèdica. Hospital Universitari de Sant Joan. Institut d´Investigació Sanitària Pere Virgili (IISPV), Universitat Rovira i Virgili
  • , Gerard AragonèsAffiliated withCentre de Recerca Biomèdica. Hospital Universitari de Sant Joan. Institut d´Investigació Sanitària Pere Virgili (IISPV), Universitat Rovira i Virgili
  • , Jordi CampsAffiliated withCentre de Recerca Biomèdica. Hospital Universitari de Sant Joan. Institut d´Investigació Sanitària Pere Virgili (IISPV), Universitat Rovira i Virgili
  • , Bharti MacknessAffiliated withCentre de Recerca Biomèdica. Hospital Universitari de Sant Joan. Institut d´Investigació Sanitària Pere Virgili (IISPV), Universitat Rovira i Virgili
  • , Michael MacknessAffiliated withCentre de Recerca Biomèdica. Hospital Universitari de Sant Joan. Institut d´Investigació Sanitària Pere Virgili (IISPV), Universitat Rovira i Virgili Email author 
  • , Jorge JovenAffiliated withCentre de Recerca Biomèdica. Hospital Universitari de Sant Joan. Institut d´Investigació Sanitària Pere Virgili (IISPV), Universitat Rovira i Virgili

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

A vicious cycle between oxidation and inflammation leads to complications in a growing number of disease states. Knowledge on tissue distribution of chemokines, mediators of inflammatory response, and paraoxonases, with antioxidant and anti-inflammatory actions, may be relevant. Using immunohistochemistry and quantitative real-time PCR we have investigated the distribution of PON1, 2 and 3, CCL2, 7, 8 and 12 and the chemokine receptor CCR2 protein and mRNA in 23 tissues from C57BL/6J mice. As expected, PON1, 2 and 3, CCL2, 7, 8 and 12 and CCR2 proteins were present in the vast majority of tissues investigated. Surprisingly, mRNA for these proteins was also expressed in most of these tissues suggesting local production and the ability to respond in situ to inflammatory stimuli. The wide distribution and expression of mRNA for paraoxonases and CC-chemokines suggest a systemic, probably coordinated, role in the overall inflammatory response.

Keywords

CCR2 Chemokines Inflammation Monocyte-chemoattractant protein Paraoxonase