Journal of Molecular Histology

, Volume 41, Issue 6, pp 379–386

Tissue distribution and expression of paraoxonases and chemokines in mouse: the ubiquitous and joint localisation suggest a systemic and coordinated role

  • Fernando Rodríguez-Sanabria
  • Anna Rull
  • Raúl Beltrán-Debón
  • Gerard Aragonès
  • Jordi Camps
  • Bharti Mackness
  • Michael Mackness
  • Jorge Joven
Original Paper

DOI: 10.1007/s10735-010-9299-x

Cite this article as:
Rodríguez-Sanabria, F., Rull, A., Beltrán-Debón, R. et al. J Mol Hist (2010) 41: 379. doi:10.1007/s10735-010-9299-x

Abstract

A vicious cycle between oxidation and inflammation leads to complications in a growing number of disease states. Knowledge on tissue distribution of chemokines, mediators of inflammatory response, and paraoxonases, with antioxidant and anti-inflammatory actions, may be relevant. Using immunohistochemistry and quantitative real-time PCR we have investigated the distribution of PON1, 2 and 3, CCL2, 7, 8 and 12 and the chemokine receptor CCR2 protein and mRNA in 23 tissues from C57BL/6J mice. As expected, PON1, 2 and 3, CCL2, 7, 8 and 12 and CCR2 proteins were present in the vast majority of tissues investigated. Surprisingly, mRNA for these proteins was also expressed in most of these tissues suggesting local production and the ability to respond in situ to inflammatory stimuli. The wide distribution and expression of mRNA for paraoxonases and CC-chemokines suggest a systemic, probably coordinated, role in the overall inflammatory response.

Keywords

CCR2 Chemokines Inflammation Monocyte-chemoattractant protein Paraoxonase 

Supplementary material

10735_2010_9299_MOESM1_ESM.doc (26 kb)
Supplementary material 1 (DOC 25 kb)
10735_2010_9299_MOESM2_ESM.doc (46 kb)
Supplementary material 2 (DOC 45 kb)

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Fernando Rodríguez-Sanabria
    • 1
  • Anna Rull
    • 1
  • Raúl Beltrán-Debón
    • 1
  • Gerard Aragonès
    • 1
  • Jordi Camps
    • 1
  • Bharti Mackness
    • 1
  • Michael Mackness
    • 1
  • Jorge Joven
    • 1
  1. 1.Centre de Recerca Biomèdica. Hospital Universitari de Sant Joan. Institut d´Investigació Sanitària Pere Virgili (IISPV)Universitat Rovira i VirgiliReusSpain

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