Journal of Molecular Histology

, Volume 40, Issue 2, pp 87–97

Molecular investigations of BKCa channels and the modulatory β-subunits in porcine basilar and middle cerebral arteries

  • Helle Wulf
  • Anders Hay-Schmidt
  • Asser Nyander Poulsen
  • Dan Arne Klaerke
  • Jes Olesen
  • Inger Jansen-Olesen
Original Paper

DOI: 10.1007/s10735-009-9216-3

Cite this article as:
Wulf, H., Hay-Schmidt, A., Poulsen, A.N. et al. J Mol Hist (2009) 40: 87. doi:10.1007/s10735-009-9216-3

Abstract

Large conductance calcium-activated potassium (BKCa) channels are fundamental in the regulation of cerebral vascular basal tone. We investigated the expression of the mRNA transcripts for the BKCa channel and its modulatory β-subunits (β1–β4) in porcine basilar and middle cerebral arteries using reverse transcription polymerase chain reaction (RT-PCR) and quantitative real-time PCR. Western blotting was used to detect immunoreactivity for the porcine BKCa channel α-subunit and β-subunit proteins. The BKCa channel α-subunit RNA and protein distribution patterns were visualized using in situ hybridization and immunofluorescence studies, respectively. The study verified that the BKCa channel α-subunit is located to smooth muscle cells of porcine basilar and middle cerebral arteries. The mRNA transcript for β1-, β2- and β4-subunit were shown by RT-PCR in porcine basilar and middle cerebral arteries. However, at the protein level, only, the β1-subunit protein was found by western blotting.

Keywords

BKCa channels Porcine cerebral arteries RT-PCR Western blotting In situ hybridization Immunofluorescence 

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Helle Wulf
    • 1
  • Anders Hay-Schmidt
    • 2
  • Asser Nyander Poulsen
    • 3
  • Dan Arne Klaerke
    • 4
  • Jes Olesen
    • 1
  • Inger Jansen-Olesen
    • 1
  1. 1.Department of Neurology & Danish Headache Center, Glostrup Research Institute, Glostrup Hospital, Faculty of Health SciencesUniversity of CopenhagenGlostrupDenmark
  2. 2.Department of Neuroscience and Pharmacology, Faculty of Health SciencesUniversity of CopenhagenCopenhagenDenmark
  3. 3.Department of Animal- and Veterinary Basic Sciences, Faculty of Life SciencesUniversity of CopenhagenFrederiksbergDenmark
  4. 4.Department of Physiology and Biochemistry, IBHV, Faculty of Life SciencesUniversity of CopenhagenFrederiksbergDenmark

Personalised recommendations