Journal of Molecular Histology

, Volume 39, Issue 6, pp 585–593

TSG-6 protein expression in the pancreatic islets of NOD mice

  • M. Kvezereli
  • S. A. Michie
  • T. Yu
  • R. J. Creusot
  • M. J. Fontaine
Original Paper

DOI: 10.1007/s10735-008-9199-5

Cite this article as:
Kvezereli, M., Michie, S.A., Yu, T. et al. J Mol Hist (2008) 39: 585. doi:10.1007/s10735-008-9199-5

Abstract

The histologic hallmark of the development of type 1 diabetes (T1D) is insulitis, characterized by leukocytic infiltration of the pancreatic islets. The molecules controlling the early influx of leukocytes into the islets are poorly understood. Tumor necrosis factor α (TNFα)-stimulated gene 6 (TSG-6) is involved in inflammation, extracellular matrix formation, cell migration, and development. In the present study, we examined the expression and cellular localization of TSG-6 protein in islets of female non-obese diabetic (NOD) mice using frozen section immunofluorescence staining. Pancreata from nondiabetic (8 and 25 weeks old), prediabetic (230–280 mg/dl blood glucose) and diabetic (>300 mg/dl blood glucose) NOD mice were stained for TSG-6, insulin, CD3, CD11c, Mac3 and CD31. TSG-6 protein was detected in 67% of islets of prediabetic mice, 27% of islets of 25-week old nondiabetic mice, and less than 7% of islets of diabetic mice and 8-week old nondiabetic mice. Lastly, islet-derived TSG-6 protein was localized to the infiltrating CD3 and CD11c positive leukocytes.

Keywords

Type 1 diabetesInsulitisInflammationTSG-6Dendritic cellsT lymphocytes

Supplementary material

10735_2008_9199_MOESM1_ESM.jpg (69 kb)
MOESM1 [TSG-6 gene fragment amplified by PCR. Lane 1 shows DNA markers, Lane 2 indicates 851 bp fragment of the TSG-6 gene] (JPG 68 kb)
10735_2008_9199_MOESM2_ESM.jpg (3.4 mb)
MOESM2 [Immunoblotting of TSG-6 protein in lentiviral-transduced 293T cells. Lane 1 is 35 kDa recombinant TSG-6 protein as a positive control, Lane 2 is untransduced 293T cells, Lane 3 is 293T cells transduced with GFP only expressing-lentivirus, Lanes 4 and 5 are 293T cells transduced with TSG-6 GFP lentivirus at multiplicity of infection of 10 and 15, respectively] (JPG 3489 kb)

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • M. Kvezereli
    • 1
  • S. A. Michie
    • 1
  • T. Yu
    • 1
  • R. J. Creusot
    • 2
  • M. J. Fontaine
    • 1
  1. 1.Department of PathologyStanford University School of MedicineStanfordUSA
  2. 2.Department of MedicineStanford University School of MedicineStanfordUSA