Journal of Molecular Histology

, Volume 39, Issue 3, pp 265–273

Expression of tight junction proteins in epithelium including Ck20-positive M-like cells of human adenoids in vivo and in vitro

  • Ken-ichi Takano
  • Takashi Kojima
  • Noriko Ogasawara
  • Mitsuru Go
  • Shin Kikuchi
  • Takafumi Ninomiya
  • Makoto Kurose
  • Jun-ichi Koizumi
  • Ryuta Kamekura
  • Masaki Murata
  • Satoshi Tanaka
  • Hideki Chiba
  • Tetsuo Himi
  • Norimasa Sawada
Original Paper

DOI: 10.1007/s10735-008-9162-5

Cite this article as:
Takano, K., Kojima, T., Ogasawara, N. et al. J Mol Hist (2008) 39: 265. doi:10.1007/s10735-008-9162-5

Abstract

The human adenoid epithelium forms a continuous barrier against a wide variety of exogenous antigens. In this study, to elucidate the structures of the epithelial barrier in the human adenoid, including M-cells, we identified M-cells using an anti-cytokeratin 20 (Ck20) antibody and investigated expression of tight junction proteins in human adenoid epithelium in vivo and in vitro. In human adenoid epithelium and primary cultures, mRNAs of occludin, junctional adhesion molecule-A, ZO-1, and claudin-1, -4, -7, and -8 were detected by reverse transcription-polymerase chain reaction, whereas claudin-2 and -9 were expressed in vitro. In the epithelium in vivo, some Ck20-positive cells were randomly observed and indicated pocket-like structures, whereas Ck7 was positive in almost cells. Transmission electron microscopy revealed that Ck20-associated gold particles could be identified in M-like cells which had short microvilli and harboured the lymphocyte in the pocket-like structure. In primary cultures in vitro, Ck20-positive cells were also detected and had a function to take up fluorescent microparticles. In Ck20-positive cells in vivo and in vitro, expression of occludin, ZO-1, claudin-1 and -7 were observed at cell borders. These results indicate that the epithelial barrier of the human adenoid is stably maintained by expression of tight junction proteins in the epithelium including Ck20-positive M-like cells.

Keywords

Human adenoid Tight junctions M-cells Ck20 

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Ken-ichi Takano
    • 1
  • Takashi Kojima
    • 2
  • Noriko Ogasawara
    • 1
    • 2
  • Mitsuru Go
    • 1
  • Shin Kikuchi
    • 3
  • Takafumi Ninomiya
    • 3
  • Makoto Kurose
    • 1
    • 2
  • Jun-ichi Koizumi
    • 1
    • 2
  • Ryuta Kamekura
    • 1
    • 2
  • Masaki Murata
    • 2
  • Satoshi Tanaka
    • 2
  • Hideki Chiba
    • 2
  • Tetsuo Himi
    • 1
  • Norimasa Sawada
    • 2
  1. 1.Department of OtolaryngologySapporo Medical University School of MedicineSapporoJapan
  2. 2.Department of PathologySapporo Medical University School of MedicineSapporoJapan
  3. 3.Department of AnatomySapporo Medical University School of MedicineSapporoJapan