Journal of Molecular Histology

, Volume 38, Issue 5, pp 469–481

Temporal and functional changes in glycosaminoglycan expression during osteogenesis

  • Victor Nurcombe
  • Fuqi Jack Goh
  • Larisa M. Haupt
  • Sadasivam Murali
  • Simon M. Cool
Original Paper

DOI: 10.1007/s10735-007-9123-4

Cite this article as:
Nurcombe, V., Goh, F.J., Haupt, L.M. et al. J Mol Hist (2007) 38: 469. doi:10.1007/s10735-007-9123-4
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Abstract

Heparan sulfate proteoglycans (HSPGs) are complex and labile macromolecular moieties on the surfaces of cells that control the activities of a range of extracellular proteins, particularly those driving growth and regeneration. Here, we examine the biosynthesis of heparan sulfate (HS) sugars produced by cultured MC3T3-E1 mouse calvarial pre-osteoblast cells in order to explore the idea that changes in HS activity in turn drive phenotypic development during osteogenesis. Cells grown for 5 days under proliferating conditions were compared to cells grown for 20 days under mineralizing conditions with respect to their phenotype, the forms of HS core protein produced, and their HS sulfotransferase biosynthetic enzyme levels. RQ-PCR data was supported by the results from the purification of day 5 and day 20 HS forms by anionic exchange chromatography. The data show that cells in active growth phases produce more complex forms of sugar than cells that have become relatively quiescent during active mineralization, and that these in turn can differentially influence rates of cell growth when added exogenously back to preosteoblasts.

Keywords

Heparan sulfate Differentiation Phenotype Mineralization Proteoglycans Extracellular matrix Growth factors 

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Victor Nurcombe
    • 1
    • 2
  • Fuqi Jack Goh
    • 1
  • Larisa M. Haupt
    • 1
  • Sadasivam Murali
    • 1
  • Simon M. Cool
    • 1
    • 2
  1. 1.Stem Cell and Tissue Repair GroupInstitute of Molecular and Cell BiologySingaporeSingapore
  2. 2.Department of Orthopaedic Surgery, Yong Loo School of MedicineNational University of SingaporeSingaporeSingapore

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