Journal of Molecular Histology

, Volume 36, Issue 1, pp 45–50

Immunolocalisation of bilitranslocase in mucosecretory and parietal cells of the rat gastric mucosa

  • Vanessa Nicolin
  • Vittorio Grill
  • Fulvio Micali
  • Paola Narducci
  • Sabina Passamonti
Article

DOI: 10.1007/s10735-004-2920-0

Cite this article as:
Nicolin, V., Grill, V., Micali, F. et al. J Mol Hist (2005) 36: 45. doi:10.1007/s10735-004-2920-0

Abstract

SummaryBilitranslocase is a plasma membrane carrier localised at the vascular pole of the rat liver cell, where it mediates uptake of organic anions from the blood into the liver. This carrier is also present in the epithelium of the rat gastric mucosa, with similar molecular mass and functional properties. An immunohistochemical study reveals that both the mucus-secreting cells of the gastric pit and the H+-secreting parietal cells express bilitranslocase. These data point to a possible role of bilitranslocase and of its food-borne substrates (anthocyanins and nicotinic acid) in regulating the function and the permeability of the gastric mucosa.

Abbreviations

PBS

phosphate buffered saline solution

NGS

normal goat serum

Cy3

cyanine 3

FITC

fluorescein isothiocyanate

DAPI

4-6-diamidino-2 phenylindole

DABCO

1,4-Diazabicyclo[2.2.2.]octane

Copyright information

© Springer 2005

Authors and Affiliations

  • Vanessa Nicolin
    • 1
  • Vittorio Grill
    • 1
  • Fulvio Micali
    • 2
  • Paola Narducci
    • 1
  • Sabina Passamonti
    • 2
  1. 1.Dipartimento di Morfologia Umana NormaleUniversità degli Studi di TriesteTriesteItalia
  2. 2.Dipartimento di Biochimica Biofisica e Chimica delle MacromolecoleUniversità degli Studi di TriesteTriesteItalia