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The role of hydrogen peroxide in cadmium-inhibited root growth of rice seedlings

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Abstract

Cadmium (Cd) is readily taken up by the roots of rice seedlings, leading to growth reduction. H2O2 is a constituent of oxidative metabolism and is itself a reactive oxygen species. In this study, the participation of H2O2 in CdCl2-inhibited growth of rice roots was investigated. CdCl2 treatment increased H2O2 production in rice roots. CdCl2 treatment had no effect on the activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase, but inhibited the activity of catalase (CAT) in rice roots. CdCl2-inhibited root growth and -increased H2O2 content were lessened in the presence of diphenyleneiodonium chloride, an inhibitor of H2O2 generating NADPH oxidase. However, this stimulation of root growth in CdCl2-treated seedlings is small (about 5%). Calcium (Ca) is important in many physiological processes in plants. Attempts were also made to determine whether the action of Ca on CdCl2-inhibited growth of rice roots is associated with H2O2. CaCl2 application reduced the production of H2O2, the decrease in CAT activity, and the inhibition of root growth caused by CdCl2. The effects of CaCl2 application could be reversed by exogenous H2O2. Our results indicate that the Cd causes a decline in CAT and to a lower extent a stimulation of NADPH oxidase in rice roots, with the subsequent generation of H2O2, an agent responsible for growth inhibition.

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Abbreviations

APX:

Ascorbate peroxidase

AsA:

Ascorbic acid

CAT:

Catalase

CM-H2DCFDA:

5-(and-6)-Chloromethyl-2′,7′-dichlorodihydrofluorescin diacetate, acetyl ester

DPI:

Diphenyleneiodonium chloride

DW:

Dry weight

GR:

Glutathione reductase

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

References

  • Auh C-K, Murphy TM (1995) Plasma membrane redox enzyme is involved in the synthesis of O2 − ∙ and H2O2 by Phytophothora elicitor-stimulated rose cells. Plant Physiol 107:1241–1247

    PubMed  CAS  Google Scholar 

  • Bush DS (1995) Calcium regulation in plant cells and its role in signaling. Annu Rev Plant Physiol Plant Mol Biol 46:95–122

    Article  CAS  Google Scholar 

  • Chen SL, Kao CH (1995) Cd induced changes in proline level and peroxidase activity in roots or rice seedlings. Plant Growth Regul 17:67–71

    CAS  Google Scholar 

  • Chen ZS, Lee DY (1997) Evaluation of remediation techniques on two cadmium polluted soils in Taiwan. In: Iskandar IK, Adriano DC (eds) Remediation of soils contaminated with metals. Science Reviews, London, pp 209–223

    Google Scholar 

  • Cho U-H, Seo N-H (2005) Oxidative stress in Arabidopsis thaliana exposed to cadmium is due to hydrogen peroxide accumulation. Plant Sci 168:113–120

    Article  CAS  Google Scholar 

  • Dat J, Vandenabeele S, Vranová E, Van Montagu M, Inzé D, Van Breusegem F (2000) Dual action of the active oxygen species during plant stress responses. Cell Mol Life Sci 57:779–795

    Article  PubMed  CAS  Google Scholar 

  • Doke N (1983) Generation of superoxide anion by potato tuber protoplasts during hypersensitive response to hyphal cell wall components of Phytophora infestans and specific inhibition of the reaction by suppressors of hypersensitivity. Physiol Plant Pathol 23:359–367

    Article  CAS  Google Scholar 

  • El-Enany AE (1995) Alleviation of cadmium toxicity on maize seedlings by calcium. Biol Plant 37:93–99

    Article  CAS  Google Scholar 

  • Foster JG, Hess JL (1980) Responses of superoxide dismutase and glutathione reductase activities in cotton leaf tissue exposed to an atmosphere enriched in oxygen. Plant Physiol 166:482–487

    Article  Google Scholar 

  • Foyer CH, Noctor G (2000) Oxygen processing in photosynthesis: regulation and signaling. New Phytol 146:359–389

    Article  CAS  Google Scholar 

  • Foyer CH, Noctor G (2005) Oxidant and antioxidant signaling in plants: re-evaluation of the concept of oxidative stress in a physiological context. Plant Cell Environ 28:1056–1071

    Article  CAS  Google Scholar 

  • Fry SC (1986) Cross-linking of matrix polymers in the growing cell walls of angiosperms. Annu Rev Plant Physiol 37:165–186

    Article  CAS  Google Scholar 

  • Gardiner MG, Cleland R (1974) Peroxidase changes during the cessation of elongation in Pisum sativum stems. Phytochemistry 13:1095–1098

    Article  CAS  Google Scholar 

  • Garnier L, Simon-Plas F, Thuleau P, Angel J, Bein J, Ranjeva R, Montillet J-L (2006) Cadmium affects tobacco cells by a series of three waves of reactive oxygen species that contribute to cytotoxicity. Plant Cell Environ 29:1956–1969

    Article  PubMed  CAS  Google Scholar 

  • Gechev TS, Van Breusegem F, Stone JM, Denev I, Laloi C (2006) Reactive oxygen species as signals that modulate plant stress responses and programmed cell death. BioEssays 28:1091–1101

    Article  PubMed  CAS  Google Scholar 

  • Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48:909–930

    Article  PubMed  CAS  Google Scholar 

  • Goldberg R, Imberty A, Chu-Ba J (1986) Development of isoperoxidases along the growth gradient in the mung bean hypocotyls. Phytochemistry 25:1271–1274

    Article  CAS  Google Scholar 

  • Hsu YT, Kao CH (2007) Toxicity in leaves of rice exposed to cadmium is due to hydrogen peroxide accumulation. Plant Soil 298:231–241

    Article  CAS  Google Scholar 

  • Kato M, Shimizu S (1987) Chlorophyll metabolism in higher plants VII. Chlorophyll degradation in senescing tobacco leaves: phenolic-dependent peroxidative degradation. Can J Bot 65:729–735

    Article  CAS  Google Scholar 

  • Lin CC, Kao CH (1995) NaCl stress in rice seedlings: the influence of calcium on root growth. Bot Bull Acad Sin 36:41–45

    CAS  Google Scholar 

  • MacAdam JW, Nelson CJ, Sharp RE (1992) Peroxidase activity in the leaf elongation zone of tall fescue. I. Spatial distribution of ionically bound peroxidase activity in genotypes differing in length of the elongation zone. Plant Physiol 99:872–878

    Article  PubMed  CAS  Google Scholar 

  • Mittler R, Vanderauwera S, Gollery M, Van Breusegem F (2004) Reactive oxygen gene networks in plants. Trends Plant Sci 9:490–498

    Article  PubMed  CAS  Google Scholar 

  • Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880

    CAS  Google Scholar 

  • Noctor G, Foyer CH (1998) Ascorbate and glutathione: keeping active oxygen under control. Annu Rev Plant Physiol Plant Mol Biol 49:249–279

    Article  PubMed  CAS  Google Scholar 

  • Olmos EO, Martínez-Solano JR, Piqueras A, Hellin E (2003) Early steps in the oxidative burst induced by cadmium in cultured tobacco cells (BY-2 line). J Exp Bot 54:291–301

    Article  PubMed  CAS  Google Scholar 

  • Pál M, Horváth E, Janda T, Páldi E, Szalai G (2006) Physiological changes and defense mechanisms induced by cadmium stress in maize. J Plant Nutr Soil Sci 169:239–246

    Article  Google Scholar 

  • Paoletti F, Aldinucci D, Mocali A, Capparrini A (1986) A sensitive spectrophotometric method for the determination of superoxide dismutase activity in tissue extracts. Anal Biochem 154:536–541

    Article  PubMed  CAS  Google Scholar 

  • Perfus-Barbeoch L, Leonhardt N, Vavasseur A, Forestier C (2002) Heavy metal toxicity: cadmium permeates through calcium channels and disturbs the plant water status. Plant J 32:539–548

    Article  PubMed  CAS  Google Scholar 

  • Ranieri A, Castagna A, Scebba F, Careri M, Zagnoni I, Predieri G, Pagliari M, Sanitá di Toppi L (2005) Oxidative stress and phytochelatin characterisation in bread wheat exposed to cadmium excess. Plant Physiol Biochem 43:45–54

    Article  PubMed  CAS  Google Scholar 

  • Rodríguez-Serrano M, Romero-Puertas MC, Zabalza A, Corpas FJ, Gόmez M, del Río LA, Sandalio LM (2006) Cadmium effect on oxidative metabolism of pea (Pisum sativum L.) roots. Imaging of reactive oxygen species and nitric oxide accumulation in vivo. Plant Cell Environ 29:1532–1544

    Article  PubMed  Google Scholar 

  • Romero-Puertas MC, Palma JM, Gόmez M, del Río LA, Sandalio LM (2002) Cadmium causes the oxidative modification of proteins in pea plants. Plant Cell Environ 25:677–686

    Article  CAS  Google Scholar 

  • Romero-Puertas MC, Rodríguez-Serrano M, Corpas FJ, Gόmez M, del Río LA, Sandalio LM (2004) Cadmium-induced subcellular accumulation of O2 ∙ − and H2O2 in pea leaves. Plant Cell Environ 27:1122–1134

    Article  CAS  Google Scholar 

  • Sandalio LM, Rodríguez-Serrano M, Romero-Puertas MC, del Río LA (2008) Imaging of reactive oxygen species and nitric oxide in vivo in plant tissues. Methods Enzymol 440:397–409

    Article  PubMed  CAS  Google Scholar 

  • Sanitá di Toppi L, Gabbrielli R (1999) Response to cadmium in higher plants. Environ Exp Bot 41:105–130

    Article  Google Scholar 

  • Schopfer P (1996) Hydrogen peroxide-mediated cell-wall stiffening in vitro in maize coleoptile. Planta 199:43–49

    Article  CAS  Google Scholar 

  • Tsai Y-C, Hong C-Y, Liu L-F, Kao CH (2004) Relative importance of Na+ and Cl− in NaCl-induced antioxidant systems in roots of rice seedlings. Physiol Plant 122:86–94

    Article  CAS  Google Scholar 

  • Wang CQ, Song H (2009) Calcium protects Trifolium repens L. seedlings against cadmium stress. Plant Cell Rep 28:1341–1349

    Article  PubMed  CAS  Google Scholar 

  • White PJ (2000) Calcium channels in higher plants. Biochim Biophys Acta 1465:171–189

    Article  PubMed  CAS  Google Scholar 

  • Wronska-Nofer T, Wisniewska-Knypl J, Diubaltowska E, Wyszynska K (1999) Prooxidative and genotoxic effect of transition metals (cadmium, nickel, chromium, and vanadium) in mice. Trace Elem Electrol 16:87–922

    CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by a research grant from the National Science Council of the Republic of China (NSC 100-2313-B-002-002).

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Authors and Affiliations

  1. Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China

    Shih-Chueh Cho, Yun-Yang Chao & Ching Huei Kao

  2. Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China

    Chwan-Yang Hong

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  1. Shih-Chueh Cho
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  2. Yun-Yang Chao
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  4. Ching Huei Kao
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Correspondence to Ching Huei Kao.

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Cho, SC., Chao, YY., Hong, CY. et al. The role of hydrogen peroxide in cadmium-inhibited root growth of rice seedlings. Plant Growth Regul 66, 27–35 (2012). https://doi.org/10.1007/s10725-011-9625-7

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