Plant Growth Regulation

, 65:239

Cloning and characterization of an allene oxide cyclase, PpAOC3, in Physcomitrella patens

  • Takahiro Hashimoto
  • Kosaku Takahashi
  • Michio Sato
  • P. K. G. S. S. Bandara
  • Kensuke Nabeta
Original paper

DOI: 10.1007/s10725-011-9592-z

Cite this article as:
Hashimoto, T., Takahashi, K., Sato, M. et al. Plant Growth Regul (2011) 65: 239. doi:10.1007/s10725-011-9592-z

Abstract

Allene oxide cyclase (AOC) is a key enzyme in the octadecanoid pathway of flowering plants that synthesizes 12-oxo-phytodienoic acid (OPDA), which is a biosynthetic precursor of the signal molecule jasmonic acid (JA). A database search of the Physcomitrella patens genome revealed the presence of an AOC gene unique from the two previously reported AOC genes, PpAOC1 and PpAOC2. After cloning the identified AOC gene, designated PpAOC3, the obtained cDNA sequence (897 bp) was larger than the predicted AOC gene (765 bp) in the database because a speculated intron was not fully deleted. Although PpAOC3 did not display high similarity with AOC proteins from other species, recombinant PpAOC3 exhibited AOC activity and translocated to chloroplasts, as is observed for other AOC proteins. Notably, the expression profile of PpAOC3 differed from the other PpAOCs, as its expression in protonemata was higher than that in gametophores. Although the function of oxylipins such as OPDA and JA remains elusive in P. patens, further characterization of the enzymes in the octadecanoid pathway and the role of oxylipin will aid in the elucidation of physiological processes in this model bryophyte.

Keywords

Physcomitrella patensAllene oxide cyclaseChloroplastOctadecanoid pathway

Abbreviations

AOC

Allene oxide cyclase

AOS

Allene oxide synthase

12,13-EOT

(12,13S)-epoxy-(9Z,11E,15Z)-octadecatrienoic acid

13-HPOT

13(S)-hydroperoxy-(9Z,11E,15Z)-octadecatrienoic acid

IPTG

Isopropyl β-d-thiogalactopyranoside

JA

Jasmonic acid

LOX

Lipoxygenase

OPC-8:0

3-oxo-2[(Z)-pent-2-enyl]-cyclopentane-1-octanoic acid

OPDA

12-oxo-phytodienoic acid

OPR

OPDA reductase

Supplementary material

10725_2011_9592_MOESM1_ESM.doc (56 kb)
Supplementary material 1 (DOC 56 kb)

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Takahiro Hashimoto
    • 1
  • Kosaku Takahashi
    • 1
  • Michio Sato
    • 1
  • P. K. G. S. S. Bandara
    • 1
  • Kensuke Nabeta
    • 1
  1. 1.Division of Applied Bioscience, Research Faculty of AgricultureHokkaido UniversitySapporoJapan