Plant Growth Regulation

, Volume 63, Issue 3, pp 259–269

Tobacco plants ectopically expressing the Ammopiptanthus mongolicus AmCBL1 gene display enhanced tolerance to multiple abiotic stresses

Original Research

DOI: 10.1007/s10725-010-9523-4

Cite this article as:
Chen, JH., Sun, Y., Sun, F. et al. Plant Growth Regul (2011) 63: 259. doi:10.1007/s10725-010-9523-4

Abstract

Ca2+ is a critical component in signal transduction pathways that lead to stress gene expression in higher plants and regulates a wide range of physiological processes. The calcineurin B-like protein (CBL) family represents a unique group of calcium sensors in plants, and plays a key role in decoding calcium transients. Here, a CBL1 homolog gene, AmCBL1 (NCBI accession no. AY902246), was isolated from Ammopiptanthus mongolicus (Maxim.) by reverse transcriptase-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). Quantitative real-time PCR (QRT-PCR) experiments showed that AmCBL1 was significantly induced by drought, high salinity, heat, and CaCl2 treatments in A. mongolicus seedlings. Subcellular localization analysis suggested that AmCBL1 is a plasma membrane-localized protein. Our results suggest that AmCBL1 is not only a positive regulator of salt response but also a positive regulator of temperature stresses in A. mongolicus.

Keywords

Ammopiptanthus mongolicusAmCBL1Abiotic stressesCalcium sensor

Abbreviations

ABA

Abscisic acid

CaM

Calmodulin

CAML

Calmodulin like proteins

CBL

Calcineurin B-like

CDPK

Calcium dependent protein kinase

CIPK

CBL-interacting protein kinases

CNB

Calcineurin B

GFP

Green fluorescent protein

NCS

Neuronal calcium sensors

ORF

Open reading frame

PCR

Polymerase chain reaction

qRT-PCR

Quantitative real-time PCR

RACE

Rapid amplification cDNA ends

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.National Engineering Laboratory for Tree Breeding, College of Biological Sciences and BiotechnologyBeijing Forestry UniversityBeijingChina