Plant Growth Regulation

, Volume 61, Issue 3, pp 287–295

In vitro seed germination and cultivation of the aromatic medicinal Salvia stenophylla (Burch. ex Benth.) provides an alternative source of α-bisabolol

  • Hannibal T. Musarurwa
  • Johannes van Staden
  • Nokwanda P. Makunga
Original Research

DOI: 10.1007/s10725-010-9476-7

Cite this article as:
Musarurwa, H.T., van Staden, J. & Makunga, N.P. Plant Growth Regul (2010) 61: 287. doi:10.1007/s10725-010-9476-7

Abstract

The aromatic medicinal plant Salvia stenophylla contains α-bisabolol, making this plant an important contributor to the aromatherapy and cosmetic industries in South Africa. Due to its commercial importance, the cultivation of this plant using an in vitro system was considered. Firstly, seedlings were raised in vitro after breaking dormancy with light, smoke-water or chemical scarification treatments. Germination improved when seeds were smoke-treated or soaked in 70% (v/v) H2SO4. Vigorous plantlet regeneration was achieved when seedling explants were cultured on Murashige and Skoog (1962) medium with 5.7 μM IAA and 8.9 μM BA. The potential regeneration capacity for this protocol was estimated and over 1,000 plantlets can be produced from a single shoot (6.67 cm with 4–6 nodes) over a period of 3 months. Plants rooted easily regardless of their growth medium. This was followed by their successful rapid establishment and normal growth out of culture (75%). Finally, the volatile compounds in in vitro plants were compared to ex vitro plants via headspace solid phase microextraction linked to gas chromatography–mass spectrometry. The chemical complexity of microplants was similar to wild plants with in vitro plants continuing to produce α-bisabolol (21%) at high levels.

Keywords

α-Bisabolol Essential oil Lamiaceae Plant tissue culture Seed dormancy Smoke-induced germination 

Abbreviations

2,4-D

2,4 Dichlorophenoxyacetic acid

ANOVA

Analysis of variance

BA

N6-benzylaminopurine

HS–SPME–GC–MS

Headspace solid phase microextraction–gas chromatography–mass spectrometry

IAA

Indole-3-acetic acid

LSD

Least significant difference

MS

Murashige and Skoog (1962) medium

NAA

α-Naphthaleneacetic acid

PAR

Photosynthetically active radiation

PGR

Plant growth regulator

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Hannibal T. Musarurwa
    • 1
  • Johannes van Staden
    • 2
  • Nokwanda P. Makunga
    • 1
  1. 1.Department of Botany and ZoologyStellenbosch UniversityMatielandSouth Africa
  2. 2.Research Centre for Plant Growth and Development, School of Biological and Conservation SciencesUniversity of KwaZulu-Natal PietermaritzburgScottsvilleSouth Africa