Plant Growth Regulation

, Volume 60, Issue 2, pp 163–168

Heterologous expression of a Ammopiptanthus mongolicus late embryogenesis abundant protein gene (AmLEA) enhances Escherichia coli viability under cold and heat stress

  • Ruiling Liu
  • Meiqin Liu
  • Jie Liu
  • Yuzhen Chen
  • Yiyin Chen
  • Cunfu Lu
Original Paper

DOI: 10.1007/s10725-009-9432-6

Cite this article as:
Liu, R., Liu, M., Liu, J. et al. Plant Growth Regul (2010) 60: 163. doi:10.1007/s10725-009-9432-6

Abstract

A late embryogenesis abundant protein gene, AmLEA from Ammopiptanthus mongolicus, was introduced into Escherichia coli using the IMPACT™-TWIN system to analyze the possible function of AmLEA under heat and cold stresses. A fusion protein about 38 kD was expressed in E.coli cells harboring pTWIN-LEA after the induction of IPTG by SDS–PAGE analysis and the accumulation of the fusion protein peaked 3 h after IPTG addition when cultured at 37°C. Compared with control cells, the E. coli cells expressing AmLEA fusion protein showed improved chilling and heat resistence, illuminating the protein may play a protective role in cells under stress conditions. These results suggested the natively unstructured protein, similar to other members of LEA proteins, has high capacity for binding water and potential protective function against dehydration or action similar to the cold shock chaperones.

Keywords

Ammopiptanthus mongolicusAmLEA protein expressionHeat and cold stress

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Ruiling Liu
    • 1
  • Meiqin Liu
    • 1
  • Jie Liu
    • 1
  • Yuzhen Chen
    • 1
  • Yiyin Chen
    • 1
  • Cunfu Lu
    • 1
  1. 1.National Engineering Laboratory for Tree Breeding, College of Biological Sciences and BiotechnologyBeijing Forestry UniversityBeijingChina