Plant Growth Regulation

, Volume 58, Issue 2, pp 173–179

Abscisic acid (ABA) inhibition of lateral root formation involves endogenous ABA biosynthesis in Arachis hypogaea L.

Original Paper

DOI: 10.1007/s10725-009-9365-0

Cite this article as:
Guo, D., Liang, J. & Li, L. Plant Growth Regul (2009) 58: 173. doi:10.1007/s10725-009-9365-0

Abstract

ABA has been found to play a significant role in post-embryonic developmental in peanut seedlings. The results from the current study indicate that in the presence of exogenous 10 μmol l−1 ABA, lateral roots (LRs) number decreased and seedling development was delayed. This effect was eliminated by 25 μmol l−1 naproxen, an inhibitor of ABA biosynthesis. The Arabidopsis mutant deficient in ABA biosynthesis, nced3, displays a phenotype with more and longer LRs. We found that ABA decreased root-branching in peanut in a dose-dependent way. ABA-treated seedlings showed higher endogenous ABA levels than the control and naproxen-treated seedlings. RT-PCR results indicated that the expression of AhNCED1, a key gene in the ABA biosynthetic pathway, was significantly up-regulated by exogenous ABA in peanut. The mRNA levels of AhNCED1 began to increase 2 days after ABA treatment. The results from the current study show that ABA inhibits peanut LR development by increasing endogenous ABA contents.

Keywords

Abscisic acidArachis hypogaea L.Lateral roots9-cis-epoxycarotenoid dioxygenase (NCED)

Abbreviations

ABA

Abscisic acid

LR

Lateral root

NAPR

Naproxen

NCED

9-cis-epoxycarotenoid dioxygenase

RT-PCR

Reverse transcription PCR

1/2MS

Half-strength Murashige and Skoog media

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Guangdong Provincial Key Lab of Biotechnology for Plant DevelopmentCollege of Life Sciences, South China Normal UniversityGuangzhouPeople’s Republic of China