Plant Growth Regulation

, Volume 54, Issue 1, pp 45–54

Effects of short-term heat stress on oxidative damage and responses of antioxidant system in Lilium longiflorum

Original Paper

DOI: 10.1007/s10725-007-9227-6

Cite this article as:
Yin, H., Chen, Q. & Yi, M. Plant Growth Regul (2008) 54: 45. doi:10.1007/s10725-007-9227-6

Abstract

This paper aims to determine the changes in reactive oxygen species (ROS) and the responses of the lily (Lilium longiflorum L.) antioxidant system to short-term high temperatures. Plants were exposed to three levels of heat stress (37°C, 42°C, 47°C) for 10 h when hydrogen peroxide (H2O2) and superoxide (O2) production rate along with membrane injury indexes, and changes in antioxidants were measured. Compared with the control (20°C), electrolyte leakage and MDA concentration varied slightly after 10 h at 37°C and 42°C, while increased significantly at 47°C. During 10 h at 37°C and 42°C, antioxidant enzyme activities, such as SOD, POD, CAT, APX and GR, were stimulated and antioxidants (AsA and GSH concentrations) maintained high levels, which resulted in low levels of O2 and H2O2 concentration. However, after 10 h at 47°C, SOD, APX, GR activities and GSH concentration were similar to the controls, while POD, CAT activities and AsA concentration decreased significantly as compared with the control, concomitant with significant increase in O2 and H2O2 concentrations. In addition, such heat-induced effects on antioxidant enzymes were also confirmed by SOD and POD isoform, as Cu/ZnSOD maintained high stability under heat stress and the intensity of POD isoforms reduced with the duration of heat stress, especially at 47°C. It is concluded that in lily plants, the oxidative damage induced by heat stress was related to the changes in antioxidant enzyme activities and antioxidants.

Keywords

Lilium longiflorumHeat stressReactive oxygen speciesAntioxidant system

Abbreviations

ROS

Reactive oxygen species

O2

Superoxide radical

H2O2

Hydrogen peroxide

ERL

Relative electrolyte leakage

AsA

Ascorbic acid

GSH

Glutathione

SOD

Superoxide dismutase

POD

Peroxidase

CAT

Catalase

APX

Ascorbate peroxidase

GR

Glutathione reductase

MDA

Malondiadeyde

GST

Glutathione transferase

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  1. 1.Department of Ornamental Horticulture and Landscape Architecture China Agricultural UniversityBeijingChina