Plant Growth Regulation

, Volume 53, Issue 2, pp 107–115

Oxidative stress and antioxidant activity as the basis of senescence in chrysanthemum florets

Original Paper

DOI: 10.1007/s10725-007-9208-9

Cite this article as:
Chakrabarty, D., Chatterjee, J. & Datta, S.K. Plant Growth Regul (2007) 53: 107. doi:10.1007/s10725-007-9208-9
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Abstract

Stems of chrysanthemum (Chrysanthemum morifolium Ramat.) cv. Maghi were harvested when half of the buds showed colour and were put in distilled water at 21°C. Flowers showed visible senescence symptoms after 12–15 d. Reactive oxygen species (ROS) concentration and lipid peroxidation increased from young floret stage to the senescent stage. Activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), peroxidase (POD) and catalase (CAT) showed uniform increases from young floret through to the mature stage and thereafter, declined. Among the SOD isoforms, Fe-SOD and Cu/Zn-SOD were induced during the onset of senescence. Similarly different isoforms of APX and glutathione reductase (GR) also appeared during the senescence process. The capacity of the antioxidative defence system increased during the onset of senescence but the imbalance between ROS production and antioxidant defences ultimately led to oxidative damage. It is proposed that a decrease in the activity of a number of antioxidant enzymes that normally prevent the build up of free radicals can at least partially account for the observed senescence of chrysanthemum florets.

Keywords

Antioxidant enzymesChrysanthemumFloretsSenescenceReactive oxygen species

Abbreviations

APX

Ascorbate peroxidase

CAT

Catalase

GR

Glutathione reductase

LOX

Lipoxygenase

MDA

Malondialdehyde

G-POD

Guaiacol peroxidase

ROS

Reactive oxygen species

RWC

Relative water content

SOD

Superoxide dismutase

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Debasis Chakrabarty
    • 1
  • J. Chatterjee
    • 1
  • S. K. Datta
    • 1
  1. 1.Floriculture SectionNational Botanical Research InstituteLucknowIndia