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Genetic structure and gene flow in Beta vulgaris subspecies maritima along the Atlantic coast of France

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Abstract

Locating and quantifying genetic variation within crop wild relatives is an ongoing activity of gene banks tasked with ex situ conservation. Without detailed information about the population genetics of a species, geography often serves as a reasonable proxy for differentiation. With this in mind, this paper examines the genetic diversity and differentiation of Beta vulgaris subsp. maritima accessions collected along a well-studied latitudinal gradient along the French Atlantic coast of France as well as Corsica, for use as representative genotypes from the Mediterranean basin. The aim of this work is to identify the scale and magnitude of differentiation and diversity in this set of accessions, using both molecular and quantitative traits. We assessed clinal variation and admixture in genetic and morphometric data along this latitudinal gradient. Results from this study revealed a complex pattern of recent gene flow and immigration on a historical biogeographic structure. Our data suggest that a presumed latitudinal cline is in reality an admixed mosaic of genotypes.

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References

  • Biancardi E, Panella LW, Lewellen RT (2012) Beta maritima, the origin of beets. Springer, New York

    Google Scholar 

  • Boudry P, Mccombie H, Van Dijk H (2002) Vernalization requirement of wild beet Beta vulgaris ssp. maritima: among population variation and its adaptive significance. J Ecol 90:693–703

    Article  Google Scholar 

  • de Bock TSM (1986) The genus Beta: domestication, taxonomy and interspecific hybridization for plant breeding. Acta Horticul 182:335–343

    Google Scholar 

  • Doney DL (1992) Morphology of North French Atlantic Beta. In: Frese L (ed) International Beta genetic resources network. A report on the 2nd international Beta genetic resources workship held at the institute for crop science and plant breeding, Braunschweig, Germany, 24–28 June 1991. International Crop Network Series No. 7. IBPGR, Rome, pp 17–28

  • El Mousadik A, Petit RJ (1996) High level of genetic differentiation for allelic richness among populations of the argan tree [Argania spinosa (L.) Skeels] endemic to Morocco. Theor Appl Genet 92:832–839

    Article  PubMed  Google Scholar 

  • Evanno G, Regnaut S, Goudet J (2005) Detecting the number of clusters of individuals using the software structure: a simulation study. Mol Ecol 14:2611–2620

    Article  CAS  PubMed  Google Scholar 

  • Fievet V, Touzet P, Arnaud JF, Cuguen J (2007) Spatial analysis of nuclear and cytoplasmic DNA diversity in wild sea beet (Beta vulgaris ssp. maritima) populations: do marine currents shape the genetic structure? Mol Ecol 16:1847–1864

    Article  PubMed  Google Scholar 

  • Ford-Lloyd BV, Williams ALS, Williams JT (1975) A revision of Beta section Vulgares (Chenopodiaceae), with new light on the origin of cultivated beets. Bot J Linn Soc 71:89–102

    Article  Google Scholar 

  • Frese L, Desprez B, Ziegler D (2001) Potential of genetic resources and breeding strategies for base-broadening in Beta. In: Cooper HD, Spillane C, Hodgkin T (eds) Broadening the genetic base of crop production. FAO, IBPRGI jointly with CABI Publishing, Rome, pp 295–309

    Chapter  Google Scholar 

  • Goudet J (1995) FSTAT (Version 1.2): a computer program to calculate F-statistics. J Hered 86:485–486

    Google Scholar 

  • Gur A, Zamir D (2004) Unused natural variation can lift yield barriers in plant breeding. PLoS Biol 2:e245

    Article  PubMed  PubMed Central  Google Scholar 

  • Harlan JR (1976) Genetic resources in wild relatives of crops. Crop Sci 16:329–333

    Article  Google Scholar 

  • Hautekèete NC, Piquot Y, Van Dijk H (2002a) Life span in Beta vulgaris ssp. maritima: the effects of age at first reproduction and disturbance. J Ecol 90:508–516

    Article  Google Scholar 

  • Hautekèete NC, Piquot Y, Van Dijk H (2002b) Variation in ageing and meristemic activity in relation to flower-bud and fruit excision in the Beta species complex. New Phytol 154:575–579

    Article  Google Scholar 

  • Hewitt GM (1999) Post-glacial re-colonization of European biota. Biol J Linn Soc 68:87–112

    Article  Google Scholar 

  • Hewitt G (2000) The genetic legacy of the Quaternary ice ages. Nature 405:907–913

    Article  CAS  PubMed  Google Scholar 

  • Holsinger KE, Weir BS (2009) Genetics in geographically structured populations: defining, estimating and interpreting Fst. Nat Rev Genet 10:639–650

    Article  CAS  PubMed  Google Scholar 

  • Huelsenbeck JP, Andolfatto P (2007) Inference of population structure under a dirichlet process model. Genetics 175:1787–1802

    Article  CAS  PubMed  Google Scholar 

  • Jakobsson M, Rosenberg NA (2007) CLUMPP: a cluster matching and permutation program for dealing with label switching and multimodality in analysis of population structure. Bioinformatics 23:1801–1806

    Article  CAS  PubMed  Google Scholar 

  • Letschert JPW (1993) Beta section Beta: biogeographical patterns of variation, and taxonomy. Ph.D. Wageningen Agricultural University Papers, pp 93–1

  • Letschert JPW, Frese L (1993) Analysis of morphological variation in wild beet (Beta vulgaris L.) from Sicily. Genet Resour Crop Evol 40:15–24

    Article  Google Scholar 

  • Letschert JPW, Lange W, Frese L, van Der Berg RG (1994) Taxonomy of Beta section Beta. J Sugar Beet Res 31:69–85

    Article  Google Scholar 

  • Lewis PO, Zaykin D (2001) GDA user’s manual. Department of Ecology and Evolutionary Biology, University of Connecticut, http://lewis.eeb.uconn.edu/lewishome

  • Lockwood DR, Richards CM, Volk GM (2007) Probabilistic models for collecting genetic diversity: comparisons, caveats and limitations. Crop Sci 47:859–866

    Article  Google Scholar 

  • Manel S, Schwartz MK, Luikart G, Taberlet P (2003) Landscape genetics: combining landscape ecology and population genetics. Trends Ecol Evol 18:189–197

    Article  Google Scholar 

  • Manel S, Gaggiotti OE, Waples RS (2005) Assignment methods: matching biological questions with appropriate techniques. Trends Ecol Evol 20:136–142

    Article  PubMed  Google Scholar 

  • Maxted N, Ford-Lloyd BV, Jury SL, Kell SP, Scholten MA (2006) Towards a definition of a crop wild relative. Biodiv Conserv 15:2673–2685

    Article  Google Scholar 

  • Maxted N, Dulloo E, Ford-Lloyd BV, Iriondo JM, Jarvis A (2008) Gap analysis: a tool for complementary genetic conservation assessment. Divers Distrib 14:1018–1030

    Article  Google Scholar 

  • McCouch SR, McNally KL, Wang W, Sackville Hamilton R (2012) Genomics of gene banks: a case study in rice. Am J Bot 99:407–423

    Article  PubMed  Google Scholar 

  • McGrath JM, Trebbi D, Fenwick A, Panella L, Schulz B, Laurent V, Barnes S, Murray SC (2007) An open-source first-generation molecular genetic map from a sugarbeet × table beet cross and its extension to physical mapping. Plant Genom 47:27–44. (A supplement to Crop Science)

    Google Scholar 

  • Merimans PG, Hedrick PW (2011) Assessing population structure: Fst and related measures. Mol Ecol Res 11:5–18

    Article  Google Scholar 

  • Panella L, Lewellen RT (2007) Broadening the genetic base of sugar beet: introgression from wild relatives. Euphytica 154:382–400

    Article  Google Scholar 

  • Perrier X, Jacquemoud-Collet JP (2006) DARwin software. http://darwin.cirad.fr/Home.php. Accessed 20 Feb 2013

  • Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959

    CAS  PubMed  Google Scholar 

  • Rambaut A (2006) FigTree v1.3.1. 1.3.1 edn. http://tree.bio.ed.ac.uk/. Accessed 20 Feb 2013

  • Rambaut A, Drummond A (2009). FigTree v1. 3.1. Computer program and documentation distributed by the author at http://tree.bio.ed.ac.uk/software

  • Ramirez-Villegas J, Khoury C, Jarvis A, Debouck DG, Guarino L (2010) A gap analysis methodology for collecting crop genepools: a case study with Phaseolus beans. PLoS One 5:e13497

    Article  PubMed  PubMed Central  Google Scholar 

  • Reeves P, Panella L, Richards C (2012) Retention of agronomically important variation in germplasm core collections: implications for allele mining. Theor Appl Genet 124:1155–1171

    Article  PubMed  Google Scholar 

  • Richards CM, Brownson M, Mitchell S, Kresovich S, Panella L (2004) Length polymorphisms of simple sequence repeats in Beta vulgaris. Mol Ecol Notes 4:243–245

    Google Scholar 

  • Santoni S, Bervillè A (1995) Characterization of the nuclear ribosomal DNA units and phylogeny of Beta L. wild forms and cultivated beets. Theor Appl Genet 83:533–542

    Google Scholar 

  • Schmitt T (2007) Molecular biogeography of Europe: pleistocene cycles and postglacial trends. Frontiers Zool 4:11–24

    Article  Google Scholar 

  • Schwartz M, McKelvey K (2009) Why sampling scheme matters: the effect of sampling scheme on landscape genetic results. Conserv Genet 10:441–452

    Article  Google Scholar 

  • Slatkin M, Barton NH (1989) A comparison of three indirect methods for estimating average levels of gene flow. Evolution 43:1349–1368

    Article  Google Scholar 

  • Tanksley SD, McCouch SR (1997) Seed banks and molecular maps: unlocking genetic potential from the wild. Science 277:1063–1066

    Article  CAS  PubMed  Google Scholar 

  • Van Dijk H (2009) Evolutionary change in flowering phenology in the iteroparous herb Beta vulgaris ssp. maritima: a search for the underlying mechanisms. J Exp Bot 60:3143–3155

    Article  PubMed  Google Scholar 

  • Van Dijk H, Boudry P (1992) Genetic variation for life histories in Beta maritima. In: International Beta genetic resources network. A report on the 2nd international WBN workshop, institute for crop science and plant breeding, Braunschweig, Germany, 24–28, June, 1991. International Crop Network Series No. 7. IPGRI, Rome, pp 9–16

  • Van Dijk H, Boudry P, Mccombie H, Vernet P (1997) Flowering time in wild beet (Beta vulgaris ssp. maritima) along a latitudinal cline. Acta Oecol 18:47–60

    Article  Google Scholar 

  • Viard F, Bernard J, Desplanque B (2002) Crop-weed interactions in the Beta vulgaris complex at a local scale: allelic diversity and gene flow within sugar beet fields. Theor Appl Genet 104:688–697

    Article  CAS  PubMed  Google Scholar 

  • Viard F, Arnaud J-F, Delescluse M, Cuguen J (2004) Tracing back seed and pollen flow within the crop-wild Beta vulgaris complex: genetic distinctiveness vs. hot spots of hybridization over a regional scale. Mol Ecol 13:1357–1364

    Article  CAS  PubMed  Google Scholar 

  • Villain S (2007) Histoire evolutive de la section Beta. Ph.D. Universite des Sciences et Technologies de Lille

  • Villain S, Touzet P, Cuguen J (2009) Reconstructing the evolutionary history of Beta section Beta with molecular data. A focus on the Canary Islands. In: Frese L, Germeier CU, Lipman E, Maggioni L (eds) Report of the 3rd joint meeting of the ECP/GR Beta working group and world Beta network, 8–10 March 2006. Tenerife, Spain. Bioversity International, Rome, Italy

  • Volk GM, Richards CM, Reilley AA, Henk AD, Forsline PL, Aldwinckle HS (2005) Ex situ conservation of vegetatively propagated species: development of a seed-based core collection for Malus sieversii. J Am Soc Hortic Sci 130:203–210

    Google Scholar 

  • Wagmann K, Hautekèete NC, Piquot Y, Meunier C, Schmitt SE, Van Dijk H (2012) Seed dormancy distribution: explanatory ecological factors. Ann Bot 110:1205–1219

    Article  PubMed  Google Scholar 

  • Waples RS, Gaggiotti O (2006) What is a population? An empirical evaluation of some genetic methods for identifying the number of gene pools and their degree of connectivity. Mol Ecol 15:1419–1439

    Article  CAS  PubMed  Google Scholar 

  • Whitlock MC, McCauley DE (1999) Indirect measures of gene flow and migration: FST[ne]1/(4Nm + 1). Heredity 82:117–125

    Article  PubMed  Google Scholar 

  • Wilson GA, Rannala B (2003) Bayesian inference of recent migration rates using multilocus genotypes. Genetics 163:1177–1191

    PubMed  Google Scholar 

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Authors and Affiliations

  1. National Center for Genetic Resources Preservation, Agricultural Research Service, United States Department of Agriculture, 1111 South Mason Street, Fort Collins, CO, 80521, USA

    Christopher M. Richards & Patrick A. Reeves

  2. Beet Sugar Development Foundation, 1701 Centre Ave., Fort Collins, CO, 80526, USA

    Ann L. Fenwick

  3. Sugarbeet Research Unit, Agricultural Research Service, United States Department of Agriculture, 1701 Centre Ave., Fort Collins, CO, 80526, USA

    Lee Panella

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  1. Christopher M. Richards
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  2. Patrick A. Reeves
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  3. Ann L. Fenwick
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  4. Lee Panella
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Correspondence to Christopher M. Richards.

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Richards, C.M., Reeves, P.A., Fenwick, A.L. et al. Genetic structure and gene flow in Beta vulgaris subspecies maritima along the Atlantic coast of France. Genet Resour Crop Evol 61, 651–662 (2014). https://doi.org/10.1007/s10722-013-0066-1

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