, Volume 56, Issue 8, pp 1183-1193
Date: 17 Jun 2009

PCR based molecular characterization of Nepenthes khasiana Hook. f.—pitcher plant

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Abstract

Nepenthes khasiana Hook. f. belonging to monotypic family Nepenthaceae is a rare, endangered, dioecious member of the carnivorous plant found in North-East India. The plant is endemic to the Indian state of Meghalaya and is distributed throughout the state from West Khasi hills to East Khasi hills, Jaintia hills and East, West to South Garo hills from 1,000 to ca. 1,500 m altitude. Multi-locus analysis using PCR based Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) markers were used for the first time to assess the genetic diversities of N. khasiana Hook. f. collected from different parts of Meghalaya. It was observed that RAPD analysis showed more polymorphism than ISSR fingerprinting in revealing genetic polymorphism in N. khasiana Hook. f. The result of cluster analysis by using UPGMA method showed that the groups based on pooled RAPD–ISSR genetic similarity were more similar than the groups based on RAPD. Furthermore, genetic similarity reveals variability within the population at Jarain of Jaintia hills, while between populations the Baghmara region differs from the others with at least 40% dissimilarity. The results show a broad range of genetic diversity within the populations of N. khasiana Hook. f.

B. S. Bhau and K. Medhi contributed equally.