Genetic Resources and Crop Evolution

, Volume 55, Issue 6, pp 869–881

Generation and exploitation of EST-derived SSR markers for assaying molecular diversity in durum wheat populations

Authors

    • International Center for Agricultural Research in the Dry Areas (ICARDA)
  • R. K. Varshney
    • International Crops Research Institute for the Semi-Arid Tropics (ICRISAT)
  • A. Graner
    • Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)
  • J. Valkoun
    • International Center for Agricultural Research in the Dry Areas (ICARDA)
Regular Article

DOI: 10.1007/s10722-007-9292-8

Cite this article as:
Chabane, K., Varshney, R.K., Graner, A. et al. Genet Resour Crop Evol (2008) 55: 869. doi:10.1007/s10722-007-9292-8

Abstract

Durum wheat [Triticum turgidum L. subsp. turgidum convar. durum (Desf.) MK] is an important cereal crop economically and nutritionally in the Central Asia and Caucasian, West Asia, and North Africa (CWANA) regions. Durum landraces and improved lines are largely grown in this region. Its genetic diversity has been studied using different molecular markers. The increasing availability of expressed sequence tags (ESTs) in wheat (Triticum aestivum) and related cereals provides a valuable resource of non-anonymous DNA markers to study durum diversity. In this study, a set of 517,319 Triticum aestivum EST sequences was employed for the identification of wheat simple sequence repeats called microsatellites (W-eSSRs) with the help of a PERL5 script called MISA. In comparison, barley microsatellites (B-eSSRs) have been used to exploit their transferability to durum wheat. Newly developed W-eSSR markers were probed on the 115 recombinant inbred lines (RIL) of the International Triticeae Mapping Initiative (ITMI) population (Opata 85 × Synthetic 7984). The polymorphic eSSRs were mapped. To examine the potential of the two types of eSSRs markers, 12 W-eSSR markers and 13 B-eSSR markers were used to fingerprint 153 wheat genotypes. Our results indicate that: (1) B-eSSRs show a high level of transferability to wheat, (2) the developed W-eSSRs are significantly polymorphic than those derived from genomic regions, (3) new W-eSSRs were identified and integrated in the ITMI genetic linkage map and, (4) B-eSSR and W-eSSRs are providing additional markers for comparative mapping following gene introgressions from wild species and carrying out evolutionary studies.

Keywords

B-eSSRsESTsGenetic diversityGenetic linkage mappingPolymorphism Information Content (PIC)W-eSSRs

Copyright information

© Springer Science+Business Media B.V. 2007