Genetic Resources and Crop Evolution

, Volume 52, Issue 3, pp 245–247

Determining genetic diversity between lines of Vigna unguiculata subspecies by AFLP and SSR markers

Authors

    • U.S. Department of AgricultureAgricultural Research Service, Plant Genetic Resources Conservation Unit
  • M. S. Hopkins
    • ARS, Plant Genetic Resources Conservation Unitformerly USDA
  • R. E. Dean
    • Plant Genetic Resources Conservation UnitUniversity of Georgia
Short Communication

DOI: 10.1007/s10722-004-6693-9

Cite this article as:
Gillaspie, A.G., Hopkins, M.S. & Dean, R.E. Genet Resour Crop Evol (2005) 52: 245. doi:10.1007/s10722-004-6693-9

Abstract

AFLP (Amplified Fragment Length Polymorphisms) and SSR (Simple Sequence Repeat) markers were utilized to assess genetic diversity and relatedness between Vigna unguiculata subspecies. Three AFLP primer combinations and 10 SSR primer sets successfully identified closely related accessions, and the presence of heterogeneity in some accessions. AFLP methodology was successful in separating different species of Vigna. However, the level of intra-subspecies variation was as great as was the interspecies variation with both marker methods. The number of markers employed was insufficient to successfully group the subspecies into distinct clades.

Keywords

DNA markerGermplasmTaxonomyVigna unguiculata L. Walp.
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© Springer 2005