Article

Genetic Resources and Crop Evolution

, Volume 52, Issue 8, pp 1111-1120

First online:

Assessing the Genetic Diversity in the International Cocoa Genebank, Trinidad (ICG,T) using Isozyme Electrophoresis and RAPD

  • O. SounigoAffiliated withCIRAD Email author 
  • , R. UmaharanAffiliated withCocoa Research Unit, University of the West Indies
  • , Y. ChristopherAffiliated withCocoa Research Unit, University of the West Indies
  • , A. SankarAffiliated withCocoa Research Unit, University of the West Indies
  • , S. RamdahinAffiliated withCocoa Research Unit, University of the West Indies

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Random amplified polymorphic DNA (RAPD) and isozyme electrophoresis (IE) techniques were used to estimate the level of genetic diversity in a sample of cacao germplasm existing at the International Cocoa Genebank, Trinidad. Twenty-six cocoa populations represented by 459 cocoa genotypes were analysed using IE and 22 populations represented by 353 cocoa genotypes were analysed using RAPD. Despite few differences in the classification of the populations, both techniques revealed three major groups: the indigenous trees, the cultivated Trinitario and the cultivated trees from Ecuador. Two-thirds of the partitioned diversity were found within populations and one-third between the populations, with both techniques.

Keywords

Genetic diversity Isozyme Electrophoresis RAPD Theobroma cacao L.