Genetic Resources and Crop Evolution

, Volume 52, Issue 8, pp 1057–1067

Microsatellites and RAPD Markers to Study Genetic Relationships Among Cowpea Breeding Lines and Local Varieties in Senegal

Article

DOI: 10.1007/s10722-004-6107-z

Cite this article as:
Diouf, D. & Hilu, K.W. Genet Resour Crop Evol (2005) 52: 1057. doi:10.1007/s10722-004-6107-z

Abstract

Genetic diversity in local cowpea varieties and breeding lines from Senegal were studied using random amplified polymorphic DNA (RAPD) and microsatellite (SSR) techniques. Among the 61 RAPD primers used, twelve show polymorphism. Fifteen of the 30 microsatellite primer pairs were polymorphic, detecting one to nine alleles per locus. The RAPD and SSR data were analyzed both separately and in combination to assess relationships among genetic lines. Although RAPD provided information on levels of genetic diversity, microsatellite markers are most effective in determining the relationship among cowpea accessions and varieties. The SSR results support the genetic diversification of cowpea in Senegal and underscore their potential in elucidating patterns of germplasm diversity of cowpea in Senegal.

Keywords

Cowpea Germplasm resource Genetic diversity RAPD Senegal SSR UPGMA Vigna unguiculata 

Abbreviations

IITA

International Institute of Tropical Agriculture

Copyright information

© Springer 2005

Authors and Affiliations

  1. 1.Laboratoire de Biotechnologies Végétales, Faculté des Sciences et TechniquesUniversité Cheikh Anta Diop
  2. 2.Department of BiologyVirginia Polytechnic Institute and State University BlacksburgVirginiaUSA