Genetic Resources and Crop Evolution

, Volume 52, Issue 5, pp 601–607

Genetic Analysis of Egyptian Date (Phoenix dactylifera L.) Accessions Using AFLP Markers

  • Ashraf M. El-Assar
  • Robert R. Krueger
  • Pachanoor S. Devanand
  • Chih-Cheng T. Chao
Article

DOI: 10.1007/s10722-004-0583-z

Cite this article as:
El-Assar, A.M., Krueger, R.R., Devanand, P.S. et al. Genet Resour Crop Evol (2005) 52: 601. doi:10.1007/s10722-004-0583-z

Abstract

Forty-seven samples of date palm (Phoenix dactylifera L.) collected from eight locations in Egypt were studied using four sets of amplified fragment length polymorphism (AFLP) markers with near infrared fluorescence labeled primers. These samples belonged to 21 named accessions and 9 of unknown pedigrees. A total of 350 bands were scored and 233 (66.6%) were polymorphic. Twenty-seven Egyptian accessions and ‘Medjool’and ‘Deglet Noor’accessions from California could beclassified into the major cluster. This major cluster may represent a major group of date palm germplasm in North Africa. There were four other clusters, each containing one or two accessions. The variety ‘Halawy’and one accession of unknown provenance were most likely from hybridization between two clusters. Six groups of accessions of which had the same names, revealed similar but not identical AFLP profiles suggesting these accessions might derive from seedlings rather thanthrough clonal offshoot propagation.

Keywords

Genetic similarity Germplasm Molecular markers Phoenix dactylifera 

Abbreviations

AFLPs

amplified fragment length polymorphism

UPGMA

unweighted pair group method of the arithmetic average

PCA

principal coordinated analysis

PCR

polymerase chain reaction

Copyright information

© Springer 2005

Authors and Affiliations

  • Ashraf M. El-Assar
    • 1
  • Robert R. Krueger
    • 2
  • Pachanoor S. Devanand
    • 3
  • Chih-Cheng T. Chao
    • 1
  1. 1.Tropical Fruit and Date Palm Research Department Horticultural Research InstituteOrmanEgypt
  2. 2.National Clonal Germplasm Repository for Citrus and DatesUSDA-ARSRiversideUSA
  3. 3.Department of Botany and Plant SciencesUniversity of California-RiversideRiverside USA